MOLECULAR-CLONING AND DNA-SEQUENCE OF THE ARABIDOPSIS-THALIANA ALCOHOL-DEHYDROGENASE GENE
MOLECULAR-CLONING AND DNA-SEQUENCE OF THE ARABIDOPSIS-THALIANA ALCOHOL-DEHYDROGENASE GENE
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DOI：
10.1073/pnas.83.5.1408
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发表时间：
1986-03-01
影响因子：
11.1
通讯作者：
MEYEROWITZ, EM
中科院分区：
文献类型：
--
作者：
CHANG, C;MEYEROWITZ, EM
拟南芥由于其基因组尺寸非常小、几乎不存在分散的中间重复DNA以及较短的生命周期，为分子遗传学提供了一个极好的实验植物系统。我们克隆并确定了来自拟南芥的单拷贝基因的核苷酸序列，该基因可能是编码乙醇脱氢酶（ADH；酒精：NAD+氧化还原酶，EC 1.1.1.1）的基因。通过与玉米 Adhl 基因探针交叉杂交从随机重组文库中分离该基因。该DNA序列含有能够编码与玉米ADH1和ADH2(379个氨基酸)相同长度并具有.apprxeq的多肽的开放阅读框。与两种玉米酶有 80% 同源性。该开放阅读框被六个内含子打断，其位置与两个玉米基因中存在的九个内含子位置中的六个是保守的。 5''和3''非翻译区的长度分别为58和204个碱基对。对真核基因表达重要的序列，例如 TATA 盒、聚腺苷酸化信号和内含子剪接位点序列都在预期位置中找到。该基因与拟南芥 ADH 蛋白的特异性厌氧诱导杂交。
Arabidopsis thaliana provides an excellent experimental plant system for molecular genetics because of its remarkably small genome size, near absence of dispersed middle repetitive DNA, and short life cycle. We have cloned and determined the nucleotide sequence of a single-copy gene from A. thaliana likely to be the gene encoding alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1). The gene was isolated from a random recombinant library by cross-hybridization with a maize Adhl gene probe. The DNA sequence contains an open reading frame capable of encoding a polypeptide the same length as maize ADH1 and ADH2 (379 amino acids) and having .apprxeq. 80% homology with both maize enzymes. This open reading frame is interrupted by six introns whose positions are conserved with six of the nine intron positions present in both maize genes. The 5'' and 3'' untranslated regions are, respectively, 58 and 204 base pairs long. Sequences important for eukaryotic gene expression such as the TATA box, polyadenylylation signal, and intron splice-site sequences are found in the expected locations. The gene hybridizes to a specific anaerobic induction of Arabidopsis ADH protein.