Arsenic-containing hydrocarbons and arsenic-containing fatty acids: Transfer across and presystemic metabolism in the Caco-2 intestinal barrier model.
Arsenic-containing hydrocarbons and arsenic-containing fatty acids: Transfer across and presystemic metabolism in the Caco-2 intestinal barrier model.
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DOI：
10.1002/mnfr.201500286
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发表时间：
2015-10
影响因子：
5.2
通讯作者：
S. Meyer;G. Raber;F. Ebert;M. Taleshi;K. Francesconi;T. Schwerdtle
中科院分区：
文献类型：
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作者：
S. Meyer;G. Raber;F. Ebert;M. Taleshi;K. Francesconi;T. Schwerdtle
含砷烃（AsHCs）和含砷脂肪酸（AsFAs）是海洋食品中天然存在的两类砷脂。毒理学数据仍然很少，无法对其对人类健康的风险进行评估。在这里，我们研究了五种砷脂在肠屏障模型中的转移和全身前代谢。方法与结果采用3种ascs和2种AsFAs建立Caco-2肠屏障模型。因此，短链ashc的渗透率可达50%。传输可能通过被动扩散发生。AsFAs显示出较低的肠道生物利用度，但相对于砷甜菜碱或砷糖，其各自的渗透性仍高出2至5倍。有趣的是，asfa在通过体外肠屏障时有效地进行了生物转化，而AsHCs则基本不变地运输到面向血液的腔室。结论AsFAs具有系统前代谢作用，转移量低于AsHCs。相比之下，AsHCs可能对人类具有高度的肠道生物利用度。由于AsHCs在体外和体内都具有很强的毒性，因此需要对实验动物进行毒性研究以及进行人体暴露评估，以评估与海产品中存在AsHCs相关的人类健康风险。
SCOPE Arsenic-containing hydrocarbons (AsHCs) and arsenic-containing fatty acids (AsFAs) represent two classes of arsenolipids occurring naturally in marine food. Toxicological data are yet scarce and an assessment regarding the risk to human health has not been possible. Here, we investigated the transfer and presystemic metabolism of five arsenolipids in an intestinal barrier model. METHODS AND RESULTS Three AsHCs and two AsFAs were applied to the Caco-2 intestinal barrier model. Thereby, the short-chain AsHCs reached up to 50% permeability. Transport is likely to occur via passive diffusion. The AsFAs showed lower intestinal bioavailability, but respective permeabilities were still two to five times higher as compared to arsenobetaine or arsenosugars. Interestingly, AsFAs were effectively biotransformed while passing the in vitro intestinal barrier, whereas AsHCs were transported to the blood-facing compartment essentially unchanged. CONCLUSION AsFAs can be presystemically metabolised and the amount of transferred arsenic is lower than that for AsHCs. In contrast, AsHCs are likely to be highly intestinally bioavailable to humans. Since AsHCs exert strong toxicity in vitro and in vivo, toxicity studies with experimental animals as well as a human exposure assessment are needed to assess the risk to human health related to the presence of AsHCs in seafood.