SURFACE PROPERTIES OF SOME NEOPLASTIC CELLS
SURFACE PROPERTIES OF SOME NEOPLASTIC CELLS
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DOI：
10.1042/bj0840513
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发表时间：
1962-01-01
影响因子：
4.1
通讯作者：
PETHICA, BA
中科院分区：
文献类型：
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作者：
BANGHAM, AD;HOLLINGSHEAD, S;PETHICA, BA
电泳研究了pH值、离子强度、阴离子和阳离子吸附对各种肿瘤细胞的影响。埃利希腹水肿瘤细胞、克莱因淋巴肉瘤和37号肉瘤的等电点pH值在3.0[长虚线]4-5。埃利希腹水肿瘤细胞表面对I离子的吸收多于Cl离子。观察到不同有机基质对肿瘤细胞移动性的影响有显著差异。特别是，10 mM浓度的安曲霉胺和Prothidium可使埃利希腹水肿瘤和肉瘤37细胞的移动性降低30[长折线]50%，而类似物（生物无活性）的影响可以忽略不计。有机碱在不同细胞膜上表现出吸附特异性。埃利希腹水细胞对Prothidium的总吸收量远远大于外膜上的吸收量。
The effect of pH, ionic strength, and of anionic and cationic adsorption on various neoplastic cells is examined electrophoretically. Isoelectric points in the pH region 3.0[long dash]4-5 were found for the Ehrlich ascites-tumour cell, Klein lymphosarcoma and sarcoma 37. The cell surface of the Ehrlich ascites-tumour cell appears to absorb I- ion more than Cl- ion. Marked differences in the effect of various organic bases on the mobility of the tumor cells was observed. In particular, Antrycide and Prothidium at a concentration of 10 mM give a reduction of 30[long dash]50% in the mobility of the cells of the Ehrlich ascites tumour and sarcoma 37, whereas the analogues (which are biologically inactive) have a negligible effect. The organic bases show adsorption specificity on different cellmembranes. The total uptake of Prothidium by Ehrlich ascites cells is much greater than the amount adsorbed on the external membrane.