Melatonin promotes sleep-like state in zebrafish
Melatonin promotes sleep-like state in zebrafish
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DOI：
10.1016/s0006-8993(01)02444-1
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发表时间：
2001-06-08
期刊：
影响因子：
2.9
通讯作者：
Danilova, NP
中科院分区：
文献类型：
--
作者：
Zhdanova, IV;Wang, SY;Danilova, NP
松果体激素褪黑激素对人类睡眠的促进作用早在几十年前就为人所知。然而，这种现象的机制仍然不清楚，主要是因为缺乏简单的、遗传上容易驯化的动物模型。我们现在报道，褪黑素促进了一种昼夜低等脊椎动物斑马鱼(Danio Rerio)的睡眠状态，这种作用是通过激活特定的褪黑素膜受体来实现的。此外，我们的数据显示，斑马鱼的睡眠状态与哺乳动物的睡眠有根本的相似之处，包括特有的姿势，对感觉刺激的唤醒阈值提高，以及休息剥夺后代偿性休息反弹，并可由常规催眠剂、安定和戊巴比妥钠诱导。综上所述，这些数据表明褪黑素在日常物种中是进化保守的睡眠促进剂，并表明斑马鱼为研究睡眠调节的分子机制和筛选新型催眠药物提供了一个有效的动物模型。(C)2001年，爱思唯尔科学公司出版。
The sleep-promoting effect of the pineal hormone melatonin in humans is known for decades. However, the mechanisms of this phenomenon remain obscure, mainly due to lack of a simple, genetically tractable, animal model. We now report that melatonin promotes sleep-like state in a diurnal lower vertebrate, zebrafish (Danio rerio), and this effect is mediated through activation of specific melatonin membrane receptors. Furthermore, our data show that the sleep-like state in zebrafish has fundamental similarities with sleep in mammals, including characteristic postures, elevated arousal threshold to sensory stimulation and a compensatory rest rebound following rest deprivation, and can be induced by conventional hypnotics, diazepam and sodium pentobarbital. Collectively, these data indicate that melatonin is evolutionary conserved sleep-promoting agent in diurnal species and suggest that zebrafish provide an efficient animal model for studying the molecular mechanisms of sleep regulation and for screening new types of hypnotic medications. (C) 2001 Published by Elsevier Science B.V.