Central role of the CNGA4 channel subunit in Ca2+-calmodulin-dependent odor adaptation
Central role of the CNGA4 channel subunit in Ca2+-calmodulin-dependent odor adaptation
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DOI：
10.1126/science.1063224
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发表时间：
2001-12-07
期刊：
影响因子：
56.9
通讯作者：
Reed, RR
中科院分区：
文献类型：
--
作者：
Munger, SD;Lane, AP;Reed, RR
异多聚体环核苷酸门控(CNG)通道在气味信号的转导和随后的适应中起着核心作用。嗅觉感受器神经元中个别亚基对天然通道功能的贡献尚不清楚。在这里，我们表明，小鼠CNGA4基因的靶向缺失，编码一个可调节的CNG亚单位，导致气味依赖适应的缺陷。CNGA4缺失小鼠离体膜斑块中的通道表现出较慢的钙-钙调蛋白介导的通道脱敏。因此，CNGA4亚基在嗅觉信号中加速了钙离子介导的负反馈，并允许该感觉系统的快速适应。
Heteromultimeric cyclic nucleotide-gated (CNG) channels play a central role in the transduction of odorant signals and subsequent adaptation. The contributions of individual subunits to native channel function in olfactory receptor neurons remain unclear. Here, we show that the targeted deletion of the mouse CNGA4 gene, which encodes a modulatory CNG subunit, results in a defect in odorant-dependent adaptation. Channels in excised membrane patches from the CNGA4 null mouse exhibited slower Ca2+-calmodulin-mediated channel desensitization. Thus, the CNGA4 subunit accelerates the Ca2+-mediated negative feedback in olfactory signaling and allows rapid adaptation in this sensory system.