Nano-silver-modified polyphosphazene nanoparticles with different morphologies: Design, synthesis, and evaluation of antibacterial activity
Nano-silver-modified polyphosphazene nanoparticles with different morphologies: Design, synthesis, and evaluation of antibacterial activity
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DOI：
10.1016/j.cclet.2023.108584
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发表时间：
2023-05
影响因子：
9.1
通讯作者：
Tairong Kuang;Linbing Deng;Sitao Shen;H. Deng;Zhisen Shen;Zhenjie Liu;Zhengping Zhao;F. Chen;Mingqiang Zhong
中科院分区：
文献类型：
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作者：
Tairong Kuang;Linbing Deng;Sitao Shen;H. Deng;Zhisen Shen;Zhenjie Liu;Zhengping Zhao;F. Chen;Mingqiang Zhong
耐药性细菌对公众健康构成严重威胁，强调了开发无耐药性的广谱抗菌剂的重要性。在众多的银系抗菌剂中，纳米银被认为是最具发展前途和综合性能的抗菌剂。探讨银纳米粒子（AgNPs）的抗菌能力及其形态变化，为开发安全、高效的抗菌剂提供了一个出发点。采用沉淀聚合法合成了三种不同形貌的纳米银修饰聚磷腈（PRV）纳米粒子。这些纳米粒子的特征在于使用各种技术，包括傅里叶变换红外光谱（FTIR），X射线衍射（XRD），扫描电子显微镜（SEM），透射电子显微镜（TEM），和热重分析（TGA）。研究了纳米粒子对大肠杆菌（Escherichia coli，E.大肠杆菌）和金黄色葡萄球菌（S.使用最小抑菌浓度（MIC）/最小杀菌浓度（MBC）测试和倒置荧光显微术来评估金黄色葡萄球菌）。我们的研究结果表明，银纳米粒子的抗菌活性可以根据其固定形式而显着变化。Ag@PRV草莓样纳米颗粒（NPs）与Ag@PRV蛋黄壳纳米颗粒和Ag@PRV线缆样纳米纤维（NF）相比表现出更高的抗菌活性。值得注意的是，所有三种类型的合成纳米颗粒表现出对革兰氏阳性细菌比革兰氏阴性细菌更强的杀菌作用。活/死细菌染色和扫描电子显微镜证明，银可以通过改变其细胞膜的渗透性来杀死细菌。这些发现为今后新型银系抗菌剂的设计和实际应用提供了有价值的见解。
Drug-resistant bacteria present a severe threat to public health, emphasizing the importance of developing broad-spectrum antibacterial agents that are free from drug resistance. Among silver-based antibacterial agents, nano-silver has been found to exhibit the most promising and comprehensive performance. The exploration of the antibacterial capacity and morphological changes of silver nanoparticles (AgNPs) could offer a starting point for the development of safe and efficient antibacterial agents. In this study, three types of nano-silver-modified polyphosphazene (PRV) nanoparticles with different morphologies were synthesized using precipitation polymerization. These nanoparticles were characterized using various techniques, including Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and thermogravimetric analysis (TGA). The antibacterial activity of these nanoparticles againstEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus) was assessed using minimum inhibitory concentration (MIC)/minimum bactericidal concentration (MBC) tests and inverted fluorescence microscopy. Our results revealed that the antibacterial activity of silver nanoparticles can vary significantly depending on their immobilized form. Ag@PRV Strawberry-like nanoparticles (NPs) exhibited higher antibacterial activity compared to Ag@PRV Yolk-Shell NPs and Ag@PRV Cable-like nanofibers (NFs). Notably, all three types of synthesized nanoparticles demonstrated a stronger bactericidal effect on Gram-positive bacteria than Gram-negative bacteria. Live/dead bacterial staining and scanning electron microscopy demonstrated that silver can kill bacteria by altering the permeability of their cell membranes. These findings offer valuable insights for designing and practically applying new silver-based antibacterial agents in the future.