Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries
Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries
复制标题
DOI：
10.1111/j.1600-0722.2009.00662.x
复制
发表时间：
2009-10-01
影响因子：
1.9
通讯作者：
Zanin, Iriana C. J.
中科院分区：
文献类型：
--
作者：
Lima, Juliana P. M.;Sampaio de Melo, Mary A.;Zanin, Iriana C. J.
光动力抗菌治疗（PACT）由于微生物组分的光敏化而促进细菌死亡。本研究评价了PACT对牙本质原位龋的影响。在14天的过程中，20名志愿者戴上口腔内装置，其中含有每天用40%蔗糖溶液处理10次的人牙本质板。随后，评估了与47或94 J cm-2发光二极管相关的甲苯胺蓝O的抗菌效果。治疗前后，牙本质样本的微生物总数，总链球菌，变形链球菌和乳酸杆菌进行了分析。在测试的两种能量密度下，观察到PACT的细菌计数显着减少，在47 J cm-2和94 J cm-2的照射下观察到以下值：总链球菌，3.45和5.18;变形链球菌，3.08和4.16;乳杆菌，3.24和4.66;总微生物分别为4.29和5.43。仅用94 J cm-2照射处理的对照组也对所有细菌有效。总之，PACT是有效地杀死口腔微生物存在于牙本质龋原位产生的，并可能是一个有用的技术，用于消除细菌从牙本质龋病变修复前。
Photodynamic antimicrobial therapy (PACT) promotes bacterial death as a result of the photosensitization of microbial components. This study evaluated the effect of PACT on dentine caries produced in situ. Over the course of 14 d, 20 volunteers wore intra-oral devices containing human dentine slabs that were treated 10 times daily with a 40% sucrose solution. Afterwards, the antimicrobial effect of toluidine blue O, associated with 47 or 94 J cm-2 of a light-emitting diode, was evaluated. Before and after the treatments, dentine samples were analysed with regard to the total number of microorganisms, total streptococci, mutans streptococci, and lactobacilli. Significant reductions in the bacterial count were observed for PACT with both energy densities tested, with the following values observed for 47 and 94 J cm-2 of irradiation: for total streptococci, 3.45 and 5.18; for mutans streptococci, 3.08 and 4.16; for lactobacilli, 3.24 and 4.66; and for total microorganisms, 4.29 and 5.43, respectively. The control, treated with 94 J cm-2 of irradiation alone, was also effective against all bacteria. To conclude, PACT was effective in killing oral microorganisms present in dentine caries produced in situ and may be a useful technique for eliminating bacteria from dentine carious lesions before restoration.