Plasticity of Schwann cells and pericytes in response to islet injury in mice
Plasticity of Schwann cells and pericytes in response to islet injury in mice
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DOI：
10.1007/s00125-013-2977-y
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发表时间：
2013-11-01
期刊：
影响因子：
8.2
通讯作者：
Fu, Ya-Yuan
中科院分区：
文献类型：
--
作者：
Tang, Shiue-Cheng;Chiu, Yu-Chen;Fu, Ya-Yuan
胰岛施万细胞（胶质细胞）和周细胞是微器官的附属细胞，分别位于外分泌胰腺和内皮的外部和内部边界，毗邻内分泌细胞。中枢神经系统损伤后神经胶质瘢痕的形成表现出神经胶质细胞和周细胞的可塑性。目前尚不清楚类似的细胞反应是否发生在胰岛素炎中。我们应用三维（3D）组织学对正常、注射链脲佐菌素（胶质瘤阳性对照）和NOD小鼠模型的胰岛雪旺细胞网络和周细胞进行定性和定量分析。血管彩绘与小鼠胰腺组织免疫染色相结合，揭示了胰岛雪旺细胞和周细胞及其与血管系统的关系。通过光学清除制备透明胰岛标本，以方便三维共聚焦显微镜对组织网络进行全景可视化。深度显微镜显示，胰岛雪旺细胞网络从胰岛周围延伸到核心。注射链脲佐菌素一周后，我们观察到胰岛内血管周围胶质瘤和周细胞密度增加。在NOD小鼠的早期/中度胰岛素炎中，病变周围胶质瘤发生在淋巴细胞浸润的前部，具有非典型的胰岛雪旺细胞形态，包括过度分支和血管周围胶质瘤。与此同时，周细胞聚集在供血小动脉的壁周围和局部区域，表面标记物密度显著增加。反应性细胞反应表现出可塑性，并表明当损伤发生时，由与胰岛病变和脉管系统相关的雪旺细胞和周细胞组成的权宜机制。
Islet Schwann (glial) cells and pericytes are the microorgan's accessory cells positioned at the external and internal boundaries facing the exocrine pancreas and endothelium, respectively, adjacent to the endocrine cells. Plasticity of glial cells and pericytes is shown in the glial scar formation after injury to the central nervous system. It remains unclear whether similar reactive cellular responses occur in insulitis. We applied three-dimensional (3D) histology to perform qualitative and quantitative analyses of the islet Schwann cell network and pericytes in normal, streptozotocin-injected (positive control of gliosis) and NOD mouse models.Vessel painting paired with immunostaining of mouse pancreatic tissue was used to reveal the islet Schwann cells and pericytes and their association with vasculature. Transparent islet specimens were prepared by optical clearing to facilitate 3D confocal microscopy for panoramic visualisation of the tissue networks.In-depth microscopy showed that the islet Schwann cell network extends from the peri-islet domain into the core. One week after streptozotocin injection, we observed intra-islet perivascular gliosis and an increase in pericyte density. In early/moderate insulitis in the NOD mice, perilesional gliosis occurred at the front of the lymphocytic infiltration with atypical islet Schwann cell morphologies, including excessive branching and perivascular gliosis. Meanwhile, pericytes aggregated on the walls of the feeding arteriole at the peri- and intralesional domains with a marked increase in surface marker density.The reactive cellular responses demonstrate plasticity and suggest a stop-gap mechanism consisting of the Schwann cells and pericytes in association with the islet lesion and vasculature when injury occurs.