α-tocopherol and selenium facilitate recovery from lipopolysaccharide-induced sickness in aged mice
α-tocopherol and selenium facilitate recovery from lipopolysaccharide-induced sickness in aged mice
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DOI：
10.1093/jn/135.5.1157
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发表时间：
2005-05-01
影响因子：
4.2
通讯作者：
Johnson, RW
中科院分区：
文献类型：
--
作者：
Berg, BM;Godbout, JP;Johnson, RW
老年人的免疫功能下降，增加了他们感染的脆弱性。由于抗氧化剂改善了一些与年龄相关的免疫和认知功能缺陷，我们的目标是确定饮食α-生育酚（α-T）和硒是否抑制老年小鼠LPS诱导的疾病行为。雄性BALB/c小鼠从18至21月龄喂养改良的AlN 93-M饮食，所述饮食在α-T（10、75或500 mg/kg）和硒（0.05、0.15或2 mg/kg）中为低、充足或高。通过测量一种新的青少年同种的社会探索时间来量化疾病。脂多糖治疗减少了74%的社会探索在2小时，无论饮食。到4小时时，喂食低饮食的老年小鼠的社会性降低了88%，而喂食充足和高饮食的小鼠由于LPS治疗仅显示出类似于40%的减少。喂食低饮食的小鼠比喂食高饮食的小鼠具有更大的LPS诱导的体重减轻。血浆α-T浓度和谷胱甘肽过氧化物酶（GPX）活性的增加与每个增量α-T和硒24小时后LPS处理。低饮食组小鼠的脑α-T浓度和GPX活性低于高饮食组或高饮食组。不考虑饮食，白细胞介素（IL）-6，IL-1 β，和肿瘤坏死因子（TNF）α mRNA水平升高LPS在皮质，小脑，纹状体和海马类似的3倍。因此，抗氧化剂抑制疾病的行为独立于IL-6，IL-1 β，和TNF α mRNA水平2小时后，在分析的大脑区域的LPS。综上所述，这些发现表明，膳食中摄入足够的α-T和硒可能有助于促进老年人从革兰氏阴性细菌感染中恢复。
The elderly suffer a decline in immune function that increases their vulnerability to infections. Because antioxidants improve some age-related deficits in immune and cognitive function, our goal was to determine whether dietary alpha-tocopherol (alpha-T) and selenium inhibit LPS-induced sickness behavior in aged mice. Male BALB/c mice were fed modified AlN93-M diets that were low, adequate, or high in both alpha-T (10, 75, or 500 mg/kg) and selenium (0.05, 0.15, or 2 mg/kg) from 18 to 21 mo of age. Sickness was quantified by measuring time in social exploration of a novel juvenile conspecific. The lipopolysaccharide treatment reduced social exploration by 74% at 2 h, regardless of diet. By 4 h, aged mice fed the low diet were 88% less social, whereas mice fed the adequate and high diets displayed only similar to 40% reductions due to LPS treatment. Mice fed the low diet had greater LPS-induced weight loss than mice fed the high diet. Plasma alpha-T concentration and glutathione peroxidase (GPX) activity increased with each increment in alpha-T and selenium 24 h post-LPS treatment. Brain alpha-T concentration and GPX activity were lower in mice fed the low diet than in those fed the adequate or high diet. Regardless of diet, interleukin (IL)-6, IL-1 beta, and tumor necrosis factor (TNF)alpha mRNA levels were elevated by LPS similar to 3-fold in cortex, cerebellum, striatum, and hippocampus. Thus, antioxidants inhibit sickness behavior independently of IL-6, IL-1 beta, and TNF alpha mRNA levels 2 h post-LPS in the brain regions analyzed. Taken together, these findings suggest that adequate intake of dietary alpha-T and selenium may help promote recovery from gram-negative bacterial infection in the aged.