Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs
Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs
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DOI：
10.1097/01.brs.0000221995.77177.9d
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发表时间：
2006-06-15
期刊：
影响因子：
3
通讯作者：
Alini, Mauro
中科院分区：
文献类型：
--
作者：
Antoniou, John;Mwale, Fackson;Alini, Mauro
研究设计。通过酶特异性降解来确定椎间盘髓核 (NP) 的结构完整性，以将其影响与磁共振 (MR) 信号相关联。 目的。开发定量 MR 成像作为一种准确、无创的诊断工具，以更好地了解和治疗椎间盘退变。背景数据摘要。定量 MR 分析先前已被证明不仅可以反映椎间盘基质的组成，还可以反映椎间盘基质的结构完整性。需要进一步的工作来确定结构完整性与基质成分对 MR 信号的贡献。方法。将牛尾骨 NP 注射酶或缓冲液，在 37°C 下作为静态、卸载和封闭的 3 盘节段进行孵育，并通过 1.5-Tesla MR 扫描仪进行分析以测量 MR 参数。 结果。 NP 的胶原酶降解显着降低了弛豫时间，略微降低了磁化转移比，并略微增加了表观扩散系数。通过胰蛋白酶和透明质酸酶靶向蛋白聚糖和/或透明质酸完整性并没有显着影响MR参数，除了胰蛋白酶处理后椎间盘表观扩散系数增加之外。结论。我们的结果表明，基质蛋白结构完整性的变化可以通过定量 MR 进行评估。
Study Design. The structural integrity of the nucleus pulposus (NP) of intervertebral discs was targeted by enzyme-specific degradations to correlate their effects to the magnetic resonance (MR) signal.Objective. To develop quantitative MR imaging as an accurate and noninvasive diagnostic tool to better understand and treat disc degeneration.Summary of Background Data. Quantitative MR analysis has been previously shown to reflect not only the disc matrix composition, but also the structural integrity of the disc matrix. Further work is required to identify the contribution of the structural integrity versus the matrix composition to the MR signal.Methods. The bovine coccygeal NPs were injected with either enzyme or buffer, incubated at 37 C as static, unloaded and closed 3-disc segments, and analyzed by a 1.5-Tesla MR scanner to measure MR parameters.Results. Collagenase degradation of the NP significantly decreased the relaxation times, slightly decreased the magnetization transfer ratio, and slightly increased the apparent diffusion coefficient. Targeting the proteoglycan and/or hyaluronan integrity by trypsin and hyaluronidase did not significantly affect the MR parameters, except for an increase in the apparent diffusion coefficient of the disc after trypsin treatment.Conclusions. Our results demonstrate that changes in the structural integrity of matrix proteins can be assessed by quantitative MR.