Proteomic analysis of the effects of baicalein on colorectal cancer cells

Proteomic analysis of the effects of baicalein on colorectal cancer cells
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DOI：
10.1002/pmic.201100270
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发表时间：
2012-03-01
期刊：
PROTEOMICS
影响因子：
3.4
通讯作者：
Kuo, Hsing-Chun
Kuo, Hsing-Chun
中科院分区：
生物学3区
文献类型：
--
作者：
Huang, Wen-Shih;Kuo, Yi-Hung;Kuo, Hsing-Chun

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黄芩苷是具有多种药理活性的黄酮类化合物。本研究的目的是探讨黄芩素对结直肠癌（CRC）的作用，并确定黄芩素治疗的靶点。为了更好地了解黄芩素的作用靶点，采用蛋白质组学方法对黄芩素蛋白底物进行纯化和鉴定，利用2D差分凝胶电泳（2D SDS-PAGE）技术阐明黄芩素蛋白的差异展示。本研究的结果表明黄芩苷处理结直肠癌细胞导致细胞增殖减少。结果，黄芩素处理和未处理的结直肠癌之间的差异蛋白显示被确定和验证。黄芩素处理和未处理的结直肠癌有11种差异表达蛋白。此外，我们证明黄芩素通过上调过氧化物还毒素-6 （PRDX6）的水平来抑制癌细胞增殖和减少活性氧（ROS）。在黄芩素处理的CRC细胞中，通过特异性小干扰RNA敲低PRDX6导致ROS的产生和增殖，这与黄芩素处理的细胞周期分布相反。这些结果表明，黄芩苷上调PRDX6的表达，从而减弱ROS的产生，抑制CRC细胞的生长，而黄芩苷处理对正常上皮细胞没有影响。
Baicalein is the flavonoids with multiple pharmacological activities. The aim of our study was to investigate the effects of baicalein on colorectal cancer (CRC) and to recognize the targets of baicalein treatment. To better understand baicalein's target, proteomic approaches were used to purify and identify the protein substrates using 2D difference gel electrophoresis (2D SDS-PAGE) to elucidate proteins differential display. Results from this study investigate that baicalein treatment of CRC cells results in reduced cell proliferation. As a result, differential protein displays between baicalein-treated and untreated CRC were determined and validated. There were 11 differentially expressed proteins between baicalein-treated and untreated CRC. Furthermore, we demonstrate that baicalein inhibits cancer cell proliferation and reduced reactive oxygen species (ROS) by up-regulating the levels of peroxiredoxin-6 (PRDX6). Knockdown of PRDX6 in baicalein-treated CRC cells by specific small interfering RNA resulted in ROS production and proliferation, opposite of the baicalein treatment scenario as indicated by cell cycle distribution. These results illustrate that baicalein up-regulates the expression of PRDX6, which attenuates the generation of ROS and inhibits the growth of CRC cells, whereas baicalein treatment have no effect on normal epithelial cells.