Expression profiling and functional characterization of a DREB2-type gene from Populus euphratica
Expression profiling and functional characterization of a DREB2-type gene from Populus euphratica
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胡杨 DREB2 型基因的表达谱和功能表征
DOI：
10.1016/j.bbrc.2008.11.071
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发表时间：
2009-01-16
影响因子：
3.1
通讯作者：
Yin, Weilun
中科院分区：
文献类型：
--
作者：
Chen, Jinhuan;Xia, Xinli;Yin, Weilun
从荒漠胡杨中分离到一个新的脱水反应元件结合基因，命名为PeDREB2。根据多个序列比对和系统发育特征，PeDREB2被归类为DREB家族的A-2组成员。PeDREB2的表达受寒冷、干旱和高盐度的诱导，但不受脱落酸(ABA)的诱导。PeDREB2能与DRE元件特异性结合，并在洋葱表皮细胞中瞬时表达时定位于细胞核。35S启动子驱动的PeDREB2基因的表达提高了转基因烟草的耐盐性，并且没有引起生长迟缓。这些结果表明，PeDREB2作为一种新的转录因子参与了盐胁迫的响应，可能有助于提高转基因植物的耐盐性。(C)2008 Elsevier Inc.保留所有权利。
A novel DREB (dehydration responsive element binding) gene, designated PeDREB2, was isolated from the desert-grown tree, Populus euphratica. Based on multiple sequence alignment and phylogenetic characterization, PeDREB2 was classified as an A-2 group member of the DREB family. Expression of PeDREB2 was induced by cold, drought, and high salinity, but not by abscisic acid (ABA) treatment. PeDREB2 could bind specifically to DRE elements and was targeted to the nucleus when transiently expressed in onion epidermis cells. 35S promoter-driven expression of PeDREB2 improved salt tolerance in transgenic tobacco and did not cause growth retardation. The results indicate that PeDREB2 functions as a novel transcription factor involved in the response of salt stress and might be useful in improving salt tolerance in transgenic plants. (C) 2008 Elsevier Inc. All rights reserved.