Merkel cell polyomavirus (MCPyV) strains in Japanese merkel cell carcinomas (MCC) are distinct from Caaucasian type MCPyVs: genetic variability and phyllogeeny of MCPyV genomes oobtained from Japanese MCPyV-infected MCCs
Merkel cell polyomavirus (MCPyV) strains in Japanese merkel cell carcinomas (MCC) are distinct from Caaucasian type MCPyVs: genetic variability and phyllogeeny of MCPyV genomes oobtained from Japanese MCPyV-infected MCCs
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日本默克尔细胞癌 (MCC) 中的默克尔细胞多瘤病毒 (MCPyV) 株与高加索型 MCPyV 不同：从日本 MCPyV 感染的 MCC 获得的 MCPyV 基因组的遗传变异性和系统发育
DOI：
10.1007/s11262-013-1023-y
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发表时间：
2014
期刊：
影响因子：
1.6
通讯作者：
Hayashi K
中科院分区：
文献类型：
--
作者：
Matsushita M,Iwasaki T;Kuwamoto S;Kato M;Nagata K;Murakami I;Kitamura Y;Hayashi K
大多数默克尔细胞癌(MCC)是一种罕见的侵袭性皮肤癌，具有神经内分泌特征，携带有默克尔细胞多瘤病毒(MCPyV)。血清流行病学研究表明，MCPyV在人类中的流行率很高。日本已有十多个关于MCPyV毒株的序列数据，而大多数序列数据是从居住在欧洲或欧洲血统的患者那里检测到的。对32个日本MCPyV感染的MCC的小T抗原(ST)和大T抗原(LT)两个癌基因的9个几乎完整和19个部分序列的分析表明，每个感染MCPyV的MCC都含有一个特定的MCPyV毒株，带有一些同义或沉默突变和终止密码子或缺失，但抗原性功能区没有氨基酸变化。所有终止密码子均定位于视网膜母细胞瘤蛋白结合区之后。这些日本MCPyV毒株与它们之间的亲缘关系非常密切，并产生了日本毒株的一致序列。对我们在GenBank中注册的9个序列和70个其他序列的系统发育分析表明，日本或亚洲MCPyV毒株与高加索分支形成了不同的分支，我们的9个序列和75个其他序列的系统发育树形成了3个特征分支，表明所有日本或亚洲毒株都属于优势分支。提示存在MCPyV地理相关基因分型的可能性。MCPyV变异株的基因组特征将为阐明它们的进化和生物学差异提供重要的数据库和见解。
Most of merkel cell carcinomas (MCC), a rare, aggressive skin cancer with neuroendocrine features, harbor merkel cell polyomavirus (MCPyV). Seroepidemiological studies suggested high prevalence of MCPyV in the human population. More than ten sequence data on MCPyV strains in Japan have been available, whereas most sequence data were detected from patients living in Europe or European ancestry. Analysis of nine almost complete and 19 partial sequences from two oncogenes,small T antigen(ST)andlarge T antigen(LT)genomes obtained from 32 Japanese MCPyV-infected MCC revealed that each Japanese MCPyV-infected MCC harbored a specific MCPyV strain with some synonymous or, silent mutations and stop codons or deletions, but functional domains ofT antigenhad no amino acid changes. All stop codons were localized after retinoblastoma protein-binding domain. These Japanese MCPyV strains were very closely interrelated to themselves and a consensus sequence of Japanese strain was generated. Phylogenetic analysis of our nine sequences and 70 other sequences forSTandLTgene registered in GenBank indicated that Japanese or Asian MCPyV strains formed distinct clades from Caucasian clade, and phylogenetic tree of our nine and 75 other sequences forSTgene formed characteristic three clades and showed that all Japanese or Asian strains were included in the dominant clade. These suggested the possibility of geographically related genotypes of MCPyV. The genomic characterization of MCPyV variants will provide an important database and insights for illuminating their evolutional and biological differences.