MUTATIONS THAT LEAD TO REITERATIONS IN THE CELL LINEAGES OF C-ELEGANS
MUTATIONS THAT LEAD TO REITERATIONS IN THE CELL LINEAGES OF C-ELEGANS
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DOI：
10.1016/0092-8674(81)90501-8
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
SULSTON, JE
中科院分区：
文献类型：
--
作者：
CHALFIE, M;HORVITZ, HR;SULSTON, JE
线虫秀丽隐杆线虫中的细胞来自不变的细胞谱系。 2个基因的突变，unc-86和lin-4，改变了这些谱系的多个和互斥的集合。在这些突变体中，特定的细胞重复分裂模式通常与父母或祖父母祖细胞相关。 UNC-86的作用是高度特异性的，以等效的方式改变了3种胚胎后神经细胞的谱系，它们在野生型中经历了相似的分裂模式。 LIN-4的影响更加多样化，从而导致多种类型的谱系重复术以及超乳头和幼虫特异性角质层的持续合成。给定的细胞分裂或细胞分裂模式的重复导致与野生型细胞分裂模式分裂后产生的细胞反复产生（通过光学显微镜和EM）。谱系历史与细胞命运之间的这种相关性表明，在秀丽隐杆线虫中，特定的细胞分裂序列对于特定细胞类型的产生可能是必需的。经常称为干细胞谱系的重复谱系可能是线虫和其他生物的发展的基础。野生型UNC-86和LIN-4基因作用着修饰潜在的重复性细胞谱系，当消除了1个基因的活性时，这些基因将被揭示。
Cells in the nematode Caenorhabditis elegans arise from invariant cell lineages. Mutations in 2 genes, unc-86 and lin-4, alter multiple and mutually exclusive sets of these lineages. In these mutants, particular cells repeat division patterns normally associated with their parental or grandparental progenitors. The effects of unc-86 are highly specific, altering in equivalent ways the lineages of 3 post-embryonic neuroblasts that in the wild-type undergo similar division patterns. The effects of lin-4 are more varied, resulting in a number of types of lineage reiterations as well as in supernumerary molts and the continued synthesis of larval-specific cuticle. The reiteration of a given cell division or pattern of cell divisions leads to the repeated generation of cells indistinguishable (by both light microscopy and EM) from those produced after the same division of pattern of cell divisions in the wild-type. This correlation between lineage history and cell fate suggests that, in C. elegans, a particular sequence of cell divisions may be necessary for the generation of a particular cell type. Reiterative lineages often referred to as stem cell lineages may be basic to the development of nematodes and other organisms. The wild-type unc-86 and lin-4 genes act to modify latent reiterative cell lineages, which are revealed when the activity of 1 of these genes is eliminated.