Ibuprofen rescues abnormalities in periodontal tissues in conditional presenilin 1 and presenilin 2 double knockout mice.
Ibuprofen rescues abnormalities in periodontal tissues in conditional presenilin 1 and presenilin 2 double knockout mice.
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布洛芬可挽救条件性早老素 1 和早老素 2 双基因敲除小鼠牙周组织的异常
DOI：
10.3390/ijms140918457
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发表时间：
2013-09-06
影响因子：
5.6
通讯作者：
Mei B
中科院分区：
文献类型：
--
作者：
Su J;Gu J;Dong Z;Mei B
我们使用前脑特异性条件性早老素1（PS1）和早老素2（PS2）双敲除小鼠（dKO小鼠），表现出神经退行性疾病的症状，特别是阿尔茨海默病，以研究布洛芬是否可以通过减弱炎症反应来挽救大脑和牙周组织异常。解剖下颌骨进行牙槽骨高度分析。取上颌骨固定脱钙后进行组织学观察和破骨细胞检测。从海马，皮质和牙龈的下颌切牙ELISA测量用于测定炎症介质。我们证实了牙周组织异常和炎症反应的大脑和牙周组织在幼稚的9个月和12个月大的dKO小鼠。另外两组年龄匹配的dKO小鼠连续6个月接受375 ppm布洛芬治疗，表现出牙周组织损伤明显减轻，脑和牙周组织中几种炎症相关因子减少。我们的研究结果表明，抗炎药布洛芬通过dKO小鼠脑和牙周组织中的环氧合酶（考克斯）通路显著降低炎症，然后减弱牙周组织的异常。这表明布洛芬可能是预防炎症反应引起的神经系统和牙周组织损伤的理想药物。
We used forebrain-specific conditional presenilin 1 (PS1) and presenilin 2 (PS2) double knockout mice (dKO mice) that exhibit symptoms of neurodegenerative diseases, especially Alzheimer’s disease, to investigate whether ibuprofen can rescue brain and periodontal tissue abnormalities by attenuating the inflammatory response. Mandibles were dissected for alveolar bone-height analysis. Maxillae were fixed and decalcified for histological observation and osteoclast detection. ELISA measurements from the hippocampus, cortex, and gingiva of the mandibular incisor teeth were used to assay inflammatory mediators. We confirmed periodontal tissue abnormalities and inflammatory responses in brain and periodontal tissues in naive nine- and 12-month-old dKO mice. The other two groups of age-matched dKO mice that received 375-ppm ibuprofen treatment for six consecutive months exhibited significantly attenuated damage in periodontal tissues and reduction in several inflammation-related factors in brain and periodontal tissues. Our findings showed that the anti-inflammatory drug ibuprofen significantly decreased inflammation through the cyclooxygenase (COX) pathway in brain and periodontal tissues in dKO mice, and then attenuated abnormalities in periodontal tissues. This suggests that ibuprofen could be an ideal drug for preventing both nervous system and periodontal tissue damage caused by inflammatory responses.
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DOI：
10.1902/annals.2002.7.1.102
发表时间：
2002-12-01
期刊：
Annals of periodontology
影响因子：
--
作者：
Ebersole, Jeffrey L;Cappelli, David;Novak, M John
通讯作者：
Novak, M John
影响因子：
158.5
作者：
in 't Veld, BA;Ruitenberg, A;Stricker, BHC
通讯作者：
Stricker, BHC
影响因子：
16.2
作者：
Saura, CA;Choi, SY;Shen, J
通讯作者：
Shen, J
DOI：
10.1111/j.1532-5415.2007.01495.x
发表时间：
2008-01-01
影响因子：
6.3
作者：
Ellefsen, Birita;Holm-Pedersen, Poul;Waldemar, Gunhild
通讯作者：
Waldemar, Gunhild
影响因子：
3.5
作者：
Hutton, M;Hardy, J
通讯作者：
Hardy, J