Upregulated circular RNA circ-102004 that promotes cell proliferation in prostate cancer
Upregulated circular RNA circ-102004 that promotes cell proliferation in prostate cancer
复制标题
上调的环状 RNA circ-102004 可促进前列腺癌细胞增殖。
DOI：
10.1016/j.ijbiomac.2018.09.076
复制
发表时间：
2019-02-01
影响因子：
8.2
通讯作者：
Li, Zhipeng
中科院分区：
文献类型：
--
作者：
Jie Si-Tu;Cai, Yi;Li, Zhipeng
目的：前列腺癌(Pca)是世界范围内影响男性的最常见癌症之一。方法：采用实时荧光定量聚合酶链式反应和免疫组织化学方法检测cic-102004在人前列腺癌组织和细胞系中的表达。用流式细胞仪检测CIRC-102004过表达的前列腺癌永生化细胞株的细胞凋亡和细胞周期状态。通过划痕实验和Transwell实验评价转基因细胞的迁移和侵袭能力。在异种移植瘤模型中对CIRC-102004进行功能评估后，通过测序、通路分析和Western blotting来探讨CIRC-102004与经典的肿瘤信号通路之间的关系。结果：在本研究中，CIRC-102004在PCa组织中的表达显著高于匹配的正常组织。在功能实验中，CIRC-102004被证明通过刺激癌细胞的迁移和侵袭在前列腺癌中发挥致癌作用。CIRC-102004过表达还伴随着ERK、JNK和Hedgehog等多种信号通路的显著改变，这些信号通路可导致不同类型的肿瘤。结论：CIRC-102004是一个潜在的致癌基因，调控PCa的发生发展。本研究为以CIRC-102004为靶点进行诊断和治疗提供了科学依据。(C)2018年，由爱思唯尔出版。
Objective: Prostate cancer (PCa) is one of the most prevalent cancers affecting men worldwide. However, the biological functions of circRNAs in PCa are still largely unknown.Methods: Real-time PCR (RT-PCR) and immunohistochemistry were performed to characterize the circ-102004 expression in both human PCa tissues and cell lines. The apoptosis and cell cycle status of prostate immortalized cell lines that were overexpressed with circ-102004 by transfection was analyzed using flow cytometry. The scratch test and the Transwell assay were conducted to evaluate the ability of transfected cells to migrate and invade. RNA sequencing, pathway analysis, and Western blotting were performed to probe the associations of circ-102004 with the classical cancer signaling pathways after functionally evaluating circ-102004 in a xenograft tumor model.Results: In the present study, circ-102004 expression was found to be significantly higher in PCa samples than in the matched normal tissues. In functional experiments, circ-102004 is shown to play an oncogenic role in PCa by stimulating cancer cell migration and invasion. Circ-102004 overexpression was also accompanied by significant alterations in many signaling pathways, such as ERK, JNK, and Hedgehog, which are known to cause different types of cancers.Conclusions: Circ-102004 is a potential oncogenic gene that regulates the development and progression of PCa. This study provides a scientific basis for targeting circ-102004 for either diagnosis or therapy. (C) 2018 Published by Elsevier B.V.