HIGH-RESOLUTION ONE-DIMENSIONAL AND 2-DIMENSIONAL H-1 MRS OF HUMAN BRAIN-TUMOR AND NORMAL GLIAL-CELLS
HIGH-RESOLUTION ONE-DIMENSIONAL AND 2-DIMENSIONAL H-1 MRS OF HUMAN BRAIN-TUMOR AND NORMAL GLIAL-CELLS
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DOI：
10.1002/nbm.1940070303
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发表时间：
1994-05-01
影响因子：
2.9
通讯作者：
BOGDAHN, U
中科院分区：
文献类型：
--
作者：
KOTITSCHKE, K;JUNG, H;BOGDAHN, U
用H-1 mrs研究从活组织检查中建立的星形细胞瘤（WHO II级、III级）、胶质母细胞瘤、恶性黑色素瘤和正常胶质细胞培养物，在500 MHz （11.75 T）的H-1共振频率下，获得了高光谱分辨率的1D H-1光谱；结合二维位移相关（COSY） MRS，可以识别丙氨酸、天冬氨酸、胆碱、肌酸、谷氨酸、谷氨酰胺、次牛磺酸、肌醇、磷酸肌酸、磷酸乙醇胺、磷酸胆碱、乳酸、赖氨酸、n -乙酰天冬氨酸、牛磺酸、苏氨酸和缬氨酸的共振。给出了最显著化合物的T1弛豫时间。乳酸的T1值在450 ~ 850 ms之间。乳酸信号的强度在个体光谱之间存在差异，但在不同肿瘤标本和恶性程度之间没有相关性。结果表明，在1.3 ppm时，乳酸信号被苏氨酸和脂肪酸产生的峰所覆盖。不同肿瘤谱间、恶性程度相近的肿瘤谱间、同一肿瘤谱内胆碱信号水平存在差异。由于天冬氨酸、肌醇和谷氨酰胺/谷氨酸，在正常胶质细胞和不同肿瘤之间的1D和2D COSY光谱中发现了进一步的初步差异。这些结果表明，在体内光谱中观察到的一些差异可能归因于继发性宏观结构变化（缺氧、坏死），而不是肿瘤的固有特征。因此，体内和体外光谱之间需要进一步的相关性。
Astrocytoma (WHO grade II, III), glioblastoma, malignant melanoma, and normal glial cell cultures, established from biopsies, were investigated by H-1 MRS. At a H-1 resonance frequency of 500 MHz (11.75 T) a high spectral resolution was achieved in 1D H-1 spectra; in conjunction with 2D shift-correlated (COSY) MRS, resonances of alanine, aspartate, choline, creatine, glutamate, glutamine, hypotaurine, myo-inositol, phosphocreatine, phosphoryl-ethanolamine, phosphoryl-choline, lactate, lysine, N-acetylaspartate, taurine, threonine and valine could be identified. T1 relaxation times for the most prominent compounds are presented. T1 values of lactate ranged between 450 ms and 850 ms. The intensity of the lactate signal revealed differences between individual spectra, but exhibited no correlation between different tumor specimens or degree of malignancy. It was shown that the lactate signal at 1.3 ppm is covered by peaks arising from threonine and fatty acids. The choline signal level varied among spectra of different tumors, among tumors with similar degree of malignancy, and within the same tumor. Further preliminary differences due to aspartate, inositol and glutamine/glutamate were found in 1D and 2D COSY spectra between normal glial cells as well as different tumors. These results indicate that some differences observed in in vivo spectra may be attributable to secondary macroscopic structural changes (hypoxia, necrosis) and not to tumor inherent characteristics. Further correlation between in vivo and in vitro spectroscopy is therefore required.