Role of Milk-Derived Opioid Peptides and Proline Dipeptidyl Peptidase-4 in Autism Spectrum Disorders
Role of Milk-Derived Opioid Peptides and Proline Dipeptidyl Peptidase-4 in Autism Spectrum Disorders
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DOI：
10.3390/nu11010087
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发表时间：
2019-01-01
期刊：
影响因子：
5.9
通讯作者：
Kostyra, Elzbieta
中科院分区：
文献类型：
--
作者：
Jarmolowska, Beata;Bukalo, Marta;Kostyra, Elzbieta
酪蛋白等膳食蛋白质消化过程中释放的阿片肽被认为与自闭症的发展有关，从而导致了自闭症的阿片过量假说的宣布。本文探讨了外源性底物酪啡肽-7(BCM7)及其外源性底物--酪氨酸二肽酶-4(DPPIV；EC 3.4.14.5)在自闭症病因中的作用。我们的研究包括测定血清和尿液中DPPIV和BCM7的浓度，用酶联免疫吸附试验测定DPPIV的含量，用比色法测定DPPIV的活性。采用实时定量聚合酶链式反应(Real-time PCR，RT-PCR)方法检测了牛乳阿片肽对自闭症儿童和健康儿童外周血单个核细胞(PBMC)DPPIV基因表达的影响。我们的研究包括51名健康儿童和86名自闭症谱系障碍(ASD，ICDF84)儿童。我们发现自闭症患者血清中的BCM7浓度是对照组的1.6倍(p<；0.0001)。ASD儿童血清中DPPIV的浓度也显著高于对照组(p<；0.01)，而我们没有注意到两个研究组之间血清DPPIV酶活性的显著差异。按性别确认各分析参数之间的相关性。这项研究的灵感来自于日常饮食在缓解自闭症症状方面的临床经验。尽管如此，我们得出的结论是，乳源阿片肽和DPPIV是确定自闭症发病机制的潜在因素；进行的研究仍然有限，需要进一步研究。
Opioid peptides released during digestion of dietary proteins such as casein, were suggested to contribute to autism development, leading to the announcement of opioid excess hypothesis of autism. This paper examines role of enzyme proline dipeptidyl peptidase-4 (DPPIV; EC 3.4.14.5) and it is exogenous substrate, beta-casomorphin-7 (BCM7) in autism etiology. Our study included measurements of DPPIV and BCM7 concentrations in serum and urine, which were analyzed with ELISA assays and activity of DPPIV was measured by colorimetric test. The effect of opioid peptides from hydrolysed bovine milk on DPPIV gene expression in peripheral blood mononuclear cells (PBMC) in autistic and healthy children was determined using the Real-Time PCR (Polymerase Chain Reaction) method. Our research included 51 healthy children and 86 children diagnosed with autism spectrum disorder (ASD, ICDF84). We determined that the concentration of BCM7 in serum was significantly, 1.6-fold, higher in the ASD group than in controls (p < 0.0001). Concentration of DPPIV was found to also be significantly higher in serum from ASD children compared to the control group (p < 0.01), while we did not notice significant difference in enzymatic activity of serum DPPIV between the two study groups. We confirmed correlation according to the gender between analyzed parameters. The inspiration for this study emanated from clinical experience of the daily diet role in relieving the symptoms of autism. Despite this, we have concluded that milk-derived opioid peptides and DPPIV are potentially factors in determining the pathogenesis of autism; conducted studies are still limited and require further research.