Hybrid nanofibrous scaffolds from electrospinning of a synthetic biodegradable elastomer and urinary bladder matrix
Hybrid nanofibrous scaffolds from electrospinning of a synthetic biodegradable elastomer and urinary bladder matrix
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DOI：
10.1163/156856208784089599
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发表时间：
2008-01-01
影响因子：
3.6
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Stankus, John J.;Freytes, Donald O.;Wagner, William R.
合成材料可以静电纺丝成亚微米或纳米纤维支架，以模拟细胞外基质（ECM）的规模和结构，具有可重现的组成和适应性强的机械性能。然而，这些材料缺乏天然ECM中存在的生物活性。ECM衍生的支架含有生物活性分子，其在体内发挥模拟作用，如应用于软组织工程，但在机械性能控制方面不具有与一些合成物相同的灵活性。本研究的目的是将合成的、可生物降解的弹性体的可控性质与ECM衍生支架的固有生物活性联合收割机结合。制备了由可生物降解的聚酯-氨基甲酸酯脲（PEUU）和猪ECM支架（膀胱基质，UBM）组成的杂化电纺支架，并对其在体外和体内的生物活性和物理性质进行了表征。增加PEUU的量导致拉伸强度和断裂应变的线性增加，而UBM掺入导致体外平滑肌细胞粘附和增殖以及体外质量损失增加。皮下植入的混合支架导致增加支架降解和一个大的细胞浸润相比，单独的电纺PEUU。静电纺丝UBM/PEUU结合了其单个组分的有吸引力的生物活性和机械特性，导致具有相当大的软组织工程应用潜力的支架。
Synthetic materials can be electrospun into submicron or nanofibrous scaffolds to mimic extracellular matrix (ECM) scale and architecture with reproducible composition and adaptable mechanical properties. However, these materials lack the bioactivity present in natural ECM. ECM-derived scaffolds contain bioactive molecules that exert in vivo mimicking effects as applied for soft tissue engineering, yet do not possess the same flexibility in mechanical property control as some synthetics. The objective of the present study was to combine the controllable properties of a synthetic, biodegradable elastomer with the inherent bioactivity of an ECM derived scaffold. A hybrid electrospun scaffold composed of a biodegradable poly(ester-urethane)urea (PEUU) and a porcine ECM scaffold (urinary bladder matrix, UBM) was fabricated and characterized for its bioactive and physical properties both in vitro and in vivo. Increasing amounts of PEUU led to linear increases in both tensile strength and breaking strain while UBM incorporation led to increased in vitro smooth muscle cell adhesion and proliferation and in vitro mass loss. Subcutaneous implantation of the hybrid scaffolds resulted in increased scaffold degradation and a large cellular infiltrate when compared with electrospun PEUU alone. Electrospun UBM/PEUU combined the attractive bioactivity and mechanical features of its individual components to result in scaffolds with considerable potential for soft tissue engineering applications.