Blocking Interleukin-6 and Interleukin-8 Signaling Inhibits Cell Viability, Colony-forming Activity, and Cell Migration in Human Triple-negative Breast Cancer and Pancreatic Cancer Cells
Blocking Interleukin-6 and Interleukin-8 Signaling Inhibits Cell Viability, Colony-forming Activity, and Cell Migration in Human Triple-negative Breast Cancer and Pancreatic Cancer Cells
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DOI：
10.21873/anticanres.12983
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发表时间：
2018-11-01
影响因子：
2
通讯作者：
Lin, Jiayuh
中科院分区：
文献类型：
--
作者：
Fu, Shengling;Lin, Jiayuh
背景/目的：白细胞介素-6（IL-6）和白细胞介素-8（IL-8）在三阴性乳腺癌（TNBC）和胰腺导管腺癌（PDAC）的发生发展中起重要作用。这是首次将靶向这两种信号通路的小分子联合收割机用于治疗TNBC和PDAC细胞的实验。材料与方法：当TNBC或PDAC细胞用巴多昔芬（靶向IL-6）或雷帕霉素/SCH 527123（靶向IL-8）或其组合处理时，进行细胞活力、集落形成和细胞迁移测定。结果如下：在TNBC和PDAC细胞中，联合治疗比单一治疗对细胞活力、集落形成和细胞迁移具有更有效的抑制。结果还显示，巴多昔芬与SCH 527123的组合似乎比与瑞帕霉素的组合在抑制TNBC的细胞活力和集落形成方面更有效。结论：bazedoxifene和reparixin以及bazedoxifene和SCH 527123的新型药物组合可以为TNBC和PDAC提供更有效的治疗。
Background/Aim: Interleukin-6 (IL-6) and interleukin-8 (IL-8) play important roles in the progression of triple-negative breast cancer (TNBC) and pancreatic ductal adenocarcinoma (PDAC). This is the first experiment to combine small molecules targeting these two signaling pathways to treat TNBC and PDAC cells. Materials and Methods: Cell viability, colony formation and cell migration assays were conducted when TNBC or PDAC cells were treated with bazedoxifene (targeting IL-6) or reparixin/SCH527123 (targeting IL-8) or their combination. Results: The combined treatment had a more potent inhibition of cell viability, colony formation and cell migration than monotherapy in TNBC and PDAC cells. The results also showed that the combination of bazedoxifene with SCH527123 seemed to be more effective than that with reparixin in inhibiting cell viability and colony formation of TNBC. Conclusion: Novel drug combinations of bazedoxifene and reparixin, as well as bazedoxifene and SCH527123 may provide more effective treatments for TNBC and PDAC.