Genetic analysis of prototrophic natural variants of Candida albicans.
Genetic analysis of prototrophic natural variants of Candida albicans.
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白色念珠菌原养型自然变种的遗传分析。
DOI：
10.1093/genetics/123.4.667
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发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Scherer,S
中科院分区：
文献类型：
--
作者：
Goshorn,AK;Scherer,S
为了便于对白色念珠菌天然变种的遗传分析，我们分离到了一个显性的霉酚酸抗性突变体。利用抗真菌酚酸的营养缺陷菌，通过球体融合的方法分析了原生营养的自然变异体。根据分子和遗传标准，融合产物在许多亲本染色体上是杂合的。利用这种方法，我们发现了一种类型的形态变异是由于隐性改变，并鉴定了三个显性的5-氟胞嘧啶抗性突变体。罕见的融合产物表达隐性亲本标记。这些特殊的后代可用于连锁分析和品系构建。
To facilitate genetic analysis of Candida albicans natural variants, we have isolated a dominant mycophenolic acid-resistant mutant. Mycophenolic acid-resistant auxotrophs were used to analyze prototrophic natural variants by spheroplast fusion. The fusion products were shown to be heterozygous for many of the parental chromosomes by molecular and genetic criteria. Using this approach, we have found that one type of morphologic variation is due to a recessive change and identified three dominant 5-fluorocytosine-resistant mutants. Rare fusion products express recessive parental markers. These exceptional progeny should be useful for linkage analysis and strain construction.