Development of a Streptococcus pneumoniae keratitis model in mice.
Development of a Streptococcus pneumoniae keratitis model in mice.
复制标题
小鼠肺炎链球菌角膜炎模型的建立。
DOI：
10.1159/000229028
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发表时间：
2009
影响因子：
2.1
通讯作者：
Marquart,MaryE
中科院分区：
文献类型：
--
作者：
Moore3rd,QuincyC;McCormick,ClareC;Norcross,ErinW;Onwubiko,Chinwendu;Sanders,MelissaE;Fratkin,Jonathan;McDaniel,LarryS;O'Callaghan,RichardJ;Marquart,MaryE
背景：肺炎链球菌是细菌性角膜炎的常见病因，研究这种细菌的眼部致病机制的模型将有助于治疗肺炎球菌性角膜炎。目的：建立肺炎球菌性角膜炎小鼠模型。方法：对A/J、BALB/c或C57BL/6小鼠的角膜进行划痕和局部感染临床分离株OFS。肺炎。结果：A/J小鼠在感染后第1天[(1.96±0.61log10)个集落形成单位(CFU)]和第3天(1.41±0.71log10CFU)分别从眼部检出细菌。感染A/J小鼠后第3天SLE评分显著高于BALB/c和C57BL/6小鼠(p<；0.0001)，并随时间逐渐增加，在PI第10天达到最大值3.0 0±0.35。组织病理学检查显示感染后第7天和第10天有间质水肿和多形核白细胞涌入，第7天角膜破裂。
Background:Streptococcus pneumoniaeis a common cause of bacterial keratitis, and models to examine the ocular pathogenesis of this bacterium would aid in efforts to treat pneumococcal keratitis. The aim of this study was to establish a murine model of pneumococcal keratitis.Methods:The corneas of A/J, BALB/c or C57BL/6 mice were scratched and topically infected with a clinical strain ofS. pneumoniae. Slitlamp examination (SLE), enumeration of bacteria in the corneas and histology were performed.Results:Bacteria were recovered from the eyes of A/J mice on postinfection (PI) days 1 [1.96 ± 0.61 log10colony-forming units (CFU)] and 3 (1.41 ± 0.71 log10CFU). SLE scores were significantly higher in the infected A/J mice as compared to the BALB/c or C57BL/6 mice on PI day 3 (p < 0.0001) and steadily increased over time, reaching a maximal value of 3.00 ± 0.35 on PI day 10. Histopathology revealed stromal edema and the influx of polymorphonuclear leukocytes on PI days 7 and 10, and corneal disruption on PI day 7.Conclusions:S. pneumoniaekeratitis was established in A/J mice, but not BALB/c or C57BL/6 mice.