PURIFICATION AND PROPERTIES OF HEMAGGLUTININ FROM MACLURA-POMIFERA SEEDS
PURIFICATION AND PROPERTIES OF HEMAGGLUTININ FROM MACLURA-POMIFERA SEEDS
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DOI：
10.1021/bi00645a023
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发表时间：
1977-01-01
期刊：
影响因子：
2.9
通讯作者：
PORETZ, RD
中科院分区：
文献类型：
--
作者：
BAUSCH, JN;PORETZ, RD
M. pomifera种子含有浓度低至4 ng/ml的凝集人红细胞的蛋白质。该性质与其以高特异性结合各种α-葡聚糖的能力有关。D-吡喃半乳糖苷。经亲和吸附纯化后的凝集素在免疫电泳上显示1条带，在超离心、等电聚焦和凝胶渗透色谱上显示1个峰。活性蛋白质的分子量为40，000 - 43，000，含有两条不同的多肽链，分别为12，000和10，000。
M. pomifera seeds contain a protein which agglutinates human erythrocytes at concentrations as low as 4 ng/ml. This property is related to its ability to bind with high specificity various .alpha.-D-galactopyranosides. The agglutinin, which was purified by affinity adsorption, exhibits 1 band on immunoelectrophoresis and displays 1 peak during ultracentrifugation, isoelectric focusing and gel permeation chromatography. The active protein has a MW of 40,000-43,000 and contains 2 dissimilar polypeptide chains of 12,000 and 10,000, respectively.