Longistatin, an EF-hand Ca2+-binding protein from vector tick: identification, purification, and characterization.
Longistatin, an EF-hand Ca2+-binding protein from vector tick: identification, purification, and characterization.
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DOI：
10.1007/978-1-62703-230-8_9
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Tsuji, Naotoshi
中科院分区：
文献类型：
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作者：
Anisuzzaman;Islam, M Khyrul;Tsuji, Naotoshi
EF-hand的Ca（2+）结合基元是EF-hand蛋白的一个结构成分，在细胞的细胞质中起钙传感器和/或缓冲作用。然而，在少数特殊情况下，EF-hand蛋白从细胞分泌，并在细胞外发挥关键作用。我们已经从长角血蜱的唾液腺中鉴定出一种EF-hand Ca（2+）结合蛋白——长角血蜱。Longistatin具有未知结构的n端序列和c端两个EF-hand基序，保留了类似钙调素的规范结构。在含有钙或乙二胺四乙酸（EDTA）的SDS-PAGE凝胶电泳中，龙司他汀的迁移发生了明显的变化。重组和内源性的龙司他汀都可以用钌红染色，表明龙司他汀是一种Ca（2+）结合蛋白。
EF-hand Ca(2+)-binding motif, a structural component of the EF-hand protein, functions as a calcium sensor and/or buffer in the cytosol of the cell. However, in a few exceptional cases, the EF-hand proteins are secreted from cells and play crucial roles extracellularly. We have identified longistatin, an EF-hand Ca(2+)-binding protein, from the salivary glands of the tick, Haemaphysalis longicornis. Longistatin possesses an N-terminal sequence of unknown structure and two EF-hand motifs in the C-terminus, which conserve a calmodulin-like canonical structure. Longistatin shows distinct changes in its migration during electrophoresis through SDS-PAGE gel containing calcium or ethylenediaminetetraacetic acid (EDTA). Both recombinant and endogenous forms of longistatin can be stained with rutheninum red, demonstrating that longistatin is a Ca(2+)-binding protein.