High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates
High-performance liquid chromatography/tandem mass spectrometry assay for the determination of 1-methyl-4-phenyl pyridinium (MPP+) in brain tissue homogenates
复制标题
DOI：
10.1016/j.jneumeth.2004.02.011
复制
发表时间：
2004-08-30
影响因子：
3
通讯作者：
Shah, AJ
中科院分区：
文献类型：
--
作者：
Hows, MEP;Ashmeade, TE;Shah, AJ
开发了一种高通量液相色谱/串联质谱方法，用于定量评估脑组织样本中的1-甲基-4-苯基吡啶（MPP+）。该分离基于使用甲酸和乙腈作为移动的相的反相色谱法。使用梯度分离条件，MPP+在5分钟内解决，并使用串联质谱法在正离子电喷雾模式下检测。MPP+的检测限为1 fmol，信噪比为3：1。本实验室已常规使用该测定法测量I -甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）处理小鼠脑组织中MPP+水平，并可用于区分神经保护功效和单胺氧化酶抑制. (C)2004 Elsevier B. V.保留所有权利。
A high-throughput liquid chromatography/tandem mass spectrometry method has been developed for the quantitative assessment of 1-methyl-4-phenylpyridinium (MPP+) in brain tissue samples. This separation is based on reversed phase chromatography using formic acid and acetonitrile as the mobile phase. Using gradient separation conditions, MPP+ was resolved within 5 min and detected using tandem mass spectrometry in the positive ion electrospray mode. The limit of detection for MPP+ was found to be I fmol on column with a signal to noise ratio of 3: 1. The assay has been used routinely in our laboratory for the measurement of MPP+ levels in brain tissue from I -methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice, and can be used to distinguish neuroprotective efficacy and monoamine oxidase inhibition. (C) 2004 Elsevier B.V. All rights reserved.