Alcanivorax profundi sp. nov., isolated from deep seawater of the Mariana Trench
Alcanivorax profundi sp. nov., isolated from deep seawater of the Mariana Trench
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Alcanivorax profundi sp。
DOI：
10.1099/ijsem.0.003145
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发表时间：
2019
影响因子：
2.8
通讯作者：
Zhang Xiao-Hua
中科院分区：
文献类型：
--
作者：
Liu Jiwen;Ren Qiaomeng;Zhang Yunhui;Li Yuying;Tian Xiaorong;Wu Yanhong;Tian Jiwei;Zhang Xiao-Hua
从马里亚纳海沟1000 m深的海水样品中分离到一株革兰氏染色阴性、杆状、非运动、严格好氧的菌株，命名为MTEO 17 T。在10-45 °C（最佳，37 °C）、在0.0- 12.0%NaCl（w/v;最佳，3.0%）存在下和在pH 6.0-10.0（最佳，pH 7.0-8.0）下观察到生长。 根据16 S rRNA基因序列分析，菌株MTEO 17 T与南海食烷菌（Alcanivorax nanhaiticus）MCCC 1A 05629 T的同源性最高，为97.9%。 MTEO 17 Tan与A. nanhaiticusMCCC 1A 05629 T分别为78.98%和23.80%。 显著优势脂肪酸为C16：0、总特征8（C18：1ω 6c和/或C18：1ω 7 c）和总特征3（C16：1ω6c和/或C16：1ω 7 c）。 极性脂质包括两种磷脂酰乙醇胺、一种磷脂酰甘油、一种未鉴别的磷脂和四种未鉴别的极性脂质。菌株MTEO 17 T的DNA G+C含量为57.5%。 根据多相证据，菌株MTEO 17 T被认为是食铝菌属的一个新种，其名称为Alcanivorax profundisp。十一是建议。模式菌株为MTEO 17 T（=KCTC 52694 T =MCCC 1 K 03252 T）。
A Gram-stain-negative, rod-shaped, non-motile, strictly aerobic strain, designated as MTEO17T, was isolated from a 1000 m deep seawater sample of the Mariana Trench. Growth was observed at 10–45 °C (optimum, 37 °C), in the presence of 0.0–12.0 % NaCl (w/v; optimum, 3.0 %) and at pH 6.0–10.0 (optimum, pH 7.0–8.0). Phylogenetic analysis, based on the 16S rRNA gene sequence, revealed that strain MTEO17Tbelonged to the genusAlcanivoraxand showed the highest sequence similarity of 97.9 % toAlcanivorax nanhaiticusMCCC 1A05629T. The estimated average nucleotide identity and DNA–DNA hybridization values between strain MTEO17TandA. nanhaiticusMCCC 1A05629Twere 78.98 and 23.80 %, respectively. The significant dominant fatty acids were C16 : 0, summed feature 8 (C18 : 1ω6cand/or C18 : 1ω7c) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The polar lipids comprised two phosphatidylethanolamines, one phosphatidylglycerol, one unidentified phospholipid and four unidentified polar lipids. The DNA G+C content of strain MTEO17Twas 57.5 %. On the basis of the polyphasic evidence, strain MTEO17Tis proposed to represent a novel species of the genusAlcanivorax, for which the nameAlcanivorax profundisp. nov. is proposed. The type strain is MTEO17T(=KCTC 52694T=MCCC 1K03252T).