Detoxification of aflatoxin B1 and patulin by Enterococcus faecium strains
Detoxification of aflatoxin B1 and patulin by Enterococcus faecium strains
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DOI：
10.1016/j.ijfoodmicro.2010.03.006
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发表时间：
2010-05-15
影响因子：
5.4
通讯作者：
Boyaci, Ismail H.
中科院分区：
文献类型：
--
作者：
Topcu, Ali;Bulat, Tugba;Boyaci, Ismail H.
本研究旨在研究益生菌屎肠球菌M74和商品菌大肠杆菌对水溶液中黄曲霉毒素B-1和棒曲霉素的解毒作用。faecium EF 031.测试了细菌活力、孵育时间和培养基pH对结合能力的影响。此外，通过用磷酸盐缓冲盐水洗涤细菌-真菌毒素复合物来测定结合稳定性。M74和EF 031菌株均具有去除黄曲霉毒素B-1和棒曲霉素的能力。M74在48 h孵育期内可去除19.3 - 30.5%的黄曲霉毒素B-1和15.8 - 41.6%的展青霉素，而EF 031可去除23.4 - 37.5%的黄曲霉毒素B-1和19.5 - 45.3%的展青霉素。黄曲霉毒素B-1和棒曲霉素的去除率分别在pH 7.0和4.0时最高。发现与细菌菌株形成的黄曲霉毒素B-1和展青霉素复合物的稳定性高。细菌的活力对黄曲霉毒素B-1和棒曲霉素的解毒没有任何显著影响。E.粪便可能代表了在人类饮食和动物饲料中可能应用的新策略。(C)2010爱思唯尔有限公司版权所有。
Aim of the present study was to investigate the detoxification of aflatoxin B-1 and patulin from aqueous solution by probiotic culture of Enterococcus faecium M74 and commercial culture of E. faecium EF031. The effect of the bacterial viability, incubation time and pH of the medium on the binding ability was tested. Also, binding stability was determined by washing the bacteria-mycotoxin complexes with phosphate buffer saline. Both M74 and EF031 strains have the ability to remove aflatoxin B-1 and patulin. While M74 removes 19.3 to 30.5% of aflatoxin B-1 and 15.8 to 41.6% of patulin, EF031 removes 23.4 to 37.5% of aflatoxin B-1 and 19.5 to 45.3% of patulin throughout a 48 h incubation period. The removal of aflatoxin B-1 and patulin was highest at pH 7.0 and 4.0, respectively. The stability of the aflatoxin B-1 and patulin complexes formed with the bacterial strains was found to be high. The viability of the bacteria did not have any significant effect on the detoxification of aflatoxin B-1 and patulin. Detoxification properties of E. faecium could represent new strategies for a possible application in the human diet and animal feed. (C) 2010 Elsevier B.V. All rights reserved.