Crocin inhibits RANKL-induced osteoclast formation and bone resorption by suppressing NF-κB signaling pathway activation
Crocin inhibits RANKL-induced osteoclast formation and bone resorption by suppressing NF-κB signaling pathway activation
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DOI：
10.1016/j.imbio.2016.11.009
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发表时间：
2017-04-01
期刊：
影响因子：
2.8
通讯作者：
Jiao, Yong
中科院分区：
文献类型：
--
作者：
Fu, Lijia;Pan, Fang;Jiao, Yong
藏红花素是一种具有抗氧化和抗炎特性的膳食化合物，但其对骨吸收的影响尚未得到很好的表征。在这里，我们解决这个问题，通过检查西红花素对破骨细胞在体外的直接影响。在不存在或存在不同浓度藏红花素的情况下，RANKL（NF-κ B配体的受体激活剂）诱导小鼠骨髓源性巨噬细胞中的破骨细胞生成。此外，通过陷窝测定来评估用藏红花素处理的成熟破骨细胞的骨吸收活性。在不改变细胞活力的情况下，藏红花素显示出以剂量依赖性方式抑制破骨细胞的分化和功能。从机制上讲，RANKL诱导的NF-κ B和NFATc 1活化（破骨细胞分化和功能的关键信号通路）均受到骨髓源性巨噬细胞中藏红花素的抑制。因此，西红花苷通过直接抑制细胞内分子途径抑制破骨细胞生成，这可能有助于抗骨吸收治疗的未来发展。(C)2016年爱思唯尔有限公司。All rights reserved.
Crocin is a dietary compound with antioxidant and anti-inflammatory properties, but its effects on bone resorption have not been well characterized. Here we address this issue by examining the direct effects of crocin on osteoclast cells in vitro. Osteoclastogenesis was induced by RANKL (receptor activator of NF-kappa B ligand) in mouse bone marrow-derived macrophages in the absence or presence of crocin at various concentrations. Further, the bone resorption activity of mature osteoclast treated with crocin was assessed by pit assay. Without altering cell viability, crocin was shown to inhibit the differentiation and function of osteoclast cells in a dose-dependent manner. Mechanistically, RANKL-induced NF-kappa B and NFATc1 activation, the critical signaling pathways for osteoclast differentiation and function, were both repressed by crocin in bone marrow-derived macrophages. Thus, crocin suppresses osteoclastogenesis through direct inhibition of intracellular molecular pathways, which may contribute to future development of anti-bone resorption treatment. (C) 2016 Elsevier GmbH. All rights reserved.