Monitoring transplanted islets by high-frequency ultrasound
Monitoring transplanted islets by high-frequency ultrasound
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DOI：
10.4161/isl.3.5.17058
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发表时间：
2011-09-01
期刊：
影响因子：
2.2
通讯作者：
Unno, Michiaki
中科院分区：
文献类型：
--
作者：
Sakata, Naoaki;Kodama, Tetsuya;Unno, Michiaki
胰岛移植是改善1型糖尿病患者糖代谢控制的一种细胞替代疗法。建立方法来监测移植的胰岛，以及胰岛制备，是重要的，当进行胰岛移植。由于目前的成像技术仍然无法直接检测移植胰岛，我们提出了一种新的方法，使用高频超声（HF-US）可视化移植胰岛，并评估这些US结果和代谢参数之间的相关性。我们移植同基因（BALB/c小鼠）和异种（SD大鼠）胰岛到糖尿病小鼠的肾包膜下空间。通过HF-US检查受体小鼠，直到术后第28天（POD），虽然在整个观察期间可以通过HF-US检测到同源胰岛，但到POD 28时异种胰岛已经消失。HF-US计算的胰岛体积与移植的胰岛数量相关（R-2 = 0.31，p = 0.0003）和胰岛的代谢功能（血糖：R-2 = 0.15，p < 0.0001，血清胰岛素：结论：高频超声可作为一种有效的显像手段，用于显示肾包膜下移植模型中的胰岛肿块。它也可能是未来临床环境的一种可用模式。
Islet transplantation is a cell replacement therapy to improve glycometabolic control in type 1 diabetic patients. Establishing methods to monitor engrafted islets, as well as the islet preparation, is important when performing islet transplantation. Since current imaging techniques are still not available to directly detect transplanted islets, we propose a novel method to visualize transplanted islets using high-frequency ultrasound (HF-US), and to evaluate the correlation between these US findings and metabolic parameters.We transplanted syngeneic (BALB/c mice) and xenogeneic (SD rats) islets into the renal subcapsular space of diabetic mice. The recipient mice were examined by HF-US until post-operative day (POD) 28 and, while syngeneic islets could be detected by HF-US throughout the observational period, the xenogeneic islets had vanished by POD 28. The islet volume calculated by HF-US was correlated with the number of transplanted islets (R-2 = 0.31, p = 0.0003) and the metabolic function of islets (blood glucose: R-2 = 0.15, p < 0.0001, serum insulin: R-2 = 0.22, p < 0.0001).In conclusion, HF-US may be a useful imaging modality for visualizing the islet mass in renal subcapsular transplantation models. It may also be an available modality for clinical settings in the future.