Importance of dietary arachidonic acid for the growth, survival and stress resistance of larval European sea bass (Dicentrarchus labrax) fed high dietary docosahexaenoic and eicosapentaenoic acids
Importance of dietary arachidonic acid for the growth, survival and stress resistance of larval European sea bass (Dicentrarchus labrax) fed high dietary docosahexaenoic and eicosapentaenoic acids
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DOI：
10.1111/j.1365-2109.2010.02714.x
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发表时间：
2011-08-01
影响因子：
2
通讯作者：
Izquierdo, Marisol
中科院分区：
文献类型：
--
作者：
Atalah, Eyad;Hernandez-Cruz, Carmen Maria;Izquierdo, Marisol
花生四烯酸（ARA）与二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）一起被认为是海洋鱼类幼虫饲料中的必需脂肪酸。本研究的目的是确定的重要性，饲料中的ARA水平的幼欧洲海鲈鱼的性能，当EPA和DHA也存在于饮食中。18日龄的幼虫喂养，14天，明胶为基础的微量饲料含有以下ARA水平：0.3%，0.6%或1.2%。饲料ARA升高至1.2%与幼虫存活率呈正相关，并且特定生长率、体重和全长显着改善。花生四烯酸被有效地纳入幼虫的脂质，甚至在一个较高的比例比在饮食。ARA的积累增加并不影响DHA或EPA从饮食纳入幼虫总脂质。一个显着的正相关关系被发现之间的饮食ARA水平和处理压力后的生存，表明这种脂肪酸的重要性，在鲈鱼幼虫急性应激反应。结果显示ARA对鲈鱼幼虫的重要性，但应测试更高的饮食水平，以确定是否有ARA在鲈鱼的负面影响，如报告的其他物种。
Together with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), arachidonic acid (ARA) is being considered to be an essential fatty acid in marine fish larval diets. The objective of the present study was to determine the importance of dietary ARA levels for larval European sea bass performance, when EPA and DHA are also present in the diet. Eighteen-day-old larvae were fed, for 14 days, gelatine-based microdiets containing the following ARA levels: 0.3%, 0.6% or 1.2%. Elevation of dietary ARA up to 1.2% showed a positive correlation with larval survival and a significant improvement in the specific growth rates, body weight and total length. Arachidonic acid was efficiently incorporated into larval lipids, even at a higher proportion than that in the diets. Increased accumulation of ARA did not affect the incorporation of DHA or EPA from the diet into larval total lipids. A significant positive correlation was found between dietary ARA levels and survival after handling stress, indicating the importance of this fatty acid in sea bass larvae response to acute stressors. The results show the importance of ARA for sea bass larvae, but higher dietary levels should be tested to determine whether there is a negative effect of ARA in sea bass as reported for other species.