Tyrosine phosphatase SHP-1 is expressed higher in multisystem than in single-system Langerhans cell histiocytosis by immunohistochemistry
Tyrosine phosphatase SHP-1 is expressed higher in multisystem than in single-system Langerhans cell histiocytosis by immunohistochemistry
复制标题
免疫组化显示酪氨酸磷酸酶 SHP-1 在多系统中的表达高于单系统朗格汉斯细胞组织细胞增生症
DOI：
10.1007/s00428-011-1090-1
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发表时间：
2011
期刊：
影响因子：
3.5
通讯作者：
Yoshino T
中科院分区：
文献类型：
--
作者：
Murakami I;Oka T;Kuwamoto S;Kato M;Hayashi K;Gogusev J;Imamura T;Morimoto A;Imashuku S;Yoshino T
朗格汉斯细胞组织细胞增生症（Langerhans cell histiocytosis，LCH）是一种原因不明的朗格汉斯细胞样CD 1a阳性细胞（LCH cell）增殖性疾病。LCH可分为两种亚型：预后良好的单系统LCH（LCH-SS）和预后不良的多系统LCH（LCH-MS）。从LCH细胞的单克隆特征来看，LCH被认为是一种肿瘤性疾病。本研究旨在研究酪氨酸磷酸酶SHP-1在LCH中的表达，因为其表达水平在许多肿瘤中有不同的报道，在卵巢癌中过表达（候选癌蛋白），在胃癌、前列腺癌、恶性淋巴瘤和白血病中通过甲基化下调（推定的肿瘤抑制因子）。采用免疫组化方法比较LCH（LCH-SS = 21，LCH-MS = 12）、皮肤病性淋巴结病（DLA）（n= 9）和LCH病灶附近正常表皮LCs（n= 3）中SHP-1在LCH和LCH细胞中的表达。使用Photoshop软件半定量分析IHC结果。DLA、LCH-SS、LCH-MS和LC的平均强度评分（IS）分别为47、100、139和167（任意单位）。LCH-SS、LCH-MS和DLA之间的IS存在显著性差异（p< 0.01）。SHP-1在LCH-MS中的表达显著高于LCH-SS。SHP-1可作为LCH的进展标志物。SHP-1对淋巴结LCH和DLA的鉴别诊断也有一定价值。
Langerhans cell histiocytosis (LCH) is a proliferative disorder of Langerhans cell (LC)-like CD1a-positive cell (LCH cell) with unknown causes. LCH consists of two subtypes: single-system LCH (LCH-SS) with favorable prognosis and multisystem LCH (LCH-MS) with poor prognosis. LCH has been indicated as a neoplastic disorder from monoclonal characteristics of LCH cells. This study aimed to investigate an expression of tyrosine phosphatase SHP-1 in LCH, since its expression levels were variously reported in many tumors, overexpression in ovarian cancers (a candidate oncoprotein), and downregulation by methylation in gastric cancers, prostate cancers, malignant lymphomas, and leukemias (a putative tumor suppressor). By immunohistochemistry (IHC), the SHP-1 expression in LCs and LCH cells was compared in LCH (two subtypes: LCH-SS = 21, LCH-MS = 12), dermatopathic lymphadenopathy (DLA) (n= 9) and normal epidermal LCs (n= 3) near LCH lesion. IHC results were analyzed semiquantitatively using a Photoshop software. The mean intensity score (IS) of DLA, LCH-SS, LCH-MS, and LCs were 47, 100, 139, and 167 (in arbitrary unit), respectively. The IS had significant differences among LCH-SS, LCH-MS, and DLA (p< 0.01). SHP-1 is expressed significantly higher in LCH-MS than in LCH-SS. SHP-1 can be a progression marker of LCH. SHP-1 is also useful for differential diagnosis between LCH in lymph nodes and DLA.