Advances in the DNA Nanotechnology for the Cancer Biomarkers Analysis: Attributes and Applications.
Advances in the DNA Nanotechnology for the Cancer Biomarkers Analysis: Attributes and Applications.
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DOI：
10.1016/j.semcancer.2021.12.012
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发表时间：
2021-12
影响因子：
14.5
通讯作者：
Ling Xia;Yangyu Tang;Jie Zhang;Tian-Yu Dong;Rongxing Zhou
中科院分区：
文献类型：
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作者：
Ling Xia;Yangyu Tang;Jie Zhang;Tian-Yu Dong;Rongxing Zhou
临床最常用的方法是酶联免疫吸附测定（ELISA）和定量PCR（qPCR），其中ELISA用于蛋白质生物标志物的检测，而qPCR尤其适用于核酸生物标志物分析。这些构建的方法虽然得到了广泛的应用，但也表现出了灵敏度低、样本量大、操作复杂等固有的缺点。目前，基于DNA纳米技术已成功构建了多种癌症生物标志物检测方法，具有准确度高、快速、操作简单等优点。在这篇综述中，我们首次从分析属性的角度总结了基于DNA纳米技术的生物测定策略，并讨论了所报道的策略未来临床应用的可行性。
The most commonly used clinical methods are enzyme-linked immunosorbent assay (ELISA) and quantitative PCR (qPCR) in which ELISA was applied for the detection of protein biomarkers and qPCR was especially applied for nucleic acid biomarker analysis. Although these constructed methods have been applied in wide range, they also showed some inherent shortcomings such as low sensitivity, large sample volume and complex operations. At present, many methods have been successfully constructed on the basis of DNA nanotechnology with the merits of high accuracy, rapid and simple operation for cancer biomarkers assay. In this review, we summarized the bioassay strategies based on DNA nanotechnology from the perspective of the analytical attributes for the first time and discussed and the feasibility of the reported strategies for clinical application in the future.