Contemporary identification of previously reported novel Cryptosporidium isolates reveals Cryptosporidium bovis and the cervine genotype in sheep (Ovis aries)
Contemporary identification of previously reported novel Cryptosporidium isolates reveals Cryptosporidium bovis and the cervine genotype in sheep (Ovis aries)
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DOI：
10.1007/s00436-008-0935-2
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发表时间：
2008-04-01
影响因子：
2
通讯作者：
Chalmers, Rachel M.
中科院分区：
文献类型：
--
作者：
Elwin, Kristin;Chalmers, Rachel M.
隐孢子虫的分子特征在对微小隐孢子虫引起的人类隐孢子虫病水媒暴发进行调查期间，从绵羊(Ovis Aries)身上采集的样本表明，绵羊不是感染源。同时，在26株隐孢子虫卵囊壁蛋白基因座上发现了一种新的隐孢子虫基因型，另有10株用热休克蛋白引物扩增，但仍未鉴定。随后对小亚基核糖体DNA的研究发现，22个样本中含有鹿隐孢子虫，5个样本中含有牛隐孢子虫，4个样本中同时含有鹿型和牛隐孢子虫。
Molecular characterisation of Cryptosporidium spp. from sheep (Ovis aries), sampled during the investigation of a waterborne outbreak of human cryptosporidiosis caused by Cryptosporidium parvum, previously established that the sheep were not the source of infection. At the time, a novel Cryptosporidium genotype was identified at the Cryptosporidium oocyst wall protein locus in 26 isolates and a further ten isolates amplified with heat shock protein primers but remained unidentified. Subsequent investigation of small subunit ribosomal DNA has revealed that 22 samples contained Cryptosporidium cervine genotype, five contained Cryptosporidium bovis and four samples contained both the cervine genotype and C. bovis.