Exhaled breath volatile biomarker analysis for thyroid cancer
Exhaled breath volatile biomarker analysis for thyroid cancer
复制标题
甲状腺癌呼出气挥发性生物标志物分析
DOI：
10.1016/j.trsl.2015.01.005
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发表时间：
2015-08-01
影响因子：
7.8
通讯作者：
Li, Enyou
中科院分区：
文献类型：
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作者：
Guo, Lei;Wang, Changsong;Li, Enyou
与其他类型的癌症相比，甲状腺癌的发病率在过去几十年中在全球范围内迅速增加。近年来，人们对潜在的甲状腺癌生物标志物进行了研究，但这些生物标志物既没有特异性，也没有很好的阳性预测价值。呼出气分析是近年来发展起来的一种简便、无创的疾病筛查和诊断方法。在这项研究中，潜在的甲状腺癌生物标志物中的挥发性有机化合物（VOCs）进行了检测。收集64例经组织学证实的甲状腺疾病患者（其中乳头状甲状腺癌39例，结节性甲状腺肿25例）和32例健康志愿者的呼气。固相微萃取-气相色谱和质谱法被用来评估研究参与者呼出的VOCs。采用主成分分析和偏最小二乘判别分析的统计方法对最终数据进行处理。结节性甲状腺肿患者与正常对照组、乳头状甲状腺癌患者与正常对照组、乳头状甲状腺癌患者与结节性甲状腺肿患者之间的挥发性有机化合物差异显著，其中7、7、3种特征代谢物分别对样本分类起决定性作用。呼吸分析可能为甲状腺疾病的临床诊断提供一种新的、无创的、直接定性的方法。
Compared with other types of cancer, thyroid cancer incidence rates have increased rapidly worldwide in the past few decades. In recent years, potential thyroid cancer biomarkers have been studied, but these biomarkers have neither specificity nor good positive predictive value. Exhaled breath analysis is a recently developed convenient and noninvasive method for screening and diagnosing the disease. In this study, potential thyroid cancer biomarkers in volatile organic compounds (VOCs) were detected. Exhaled breath was collected from 64 patients with histologically confirmed cases of thyroid disease (including 39 individuals with papillary thyroid carcinoma and 25 individuals with nodular goiters) and 32 healthy volunteers. Solid-phase microextraction-gas chromatography and mass spectrometry was used to assess the exhaled VOCs of the study participants. The statistical methods of principal component analysis and partial least-squares discriminant analysis were performed to process the final data. The VOCs exhibited significant differences between nodular goiter patients and normal controls, papillary thyroid carcinoma patients and normal controls, and papillary thyroid carcinoma patients and nodular goiter patients; 7, 7, and 3 characteristic metabolites played decisive roles in sample classification, respectively. Breath analysis may provide a new, noninvasive, and directly qualitative method for the clinical diagnosis of thyroid disease.