REORIENTATION OF CORTICAL MICROTUBULES IN THE SUB-APICAL REGION DURING TUBERIZATION IN SINGLE-NODE STEM SEGMENTS OF POTATO IN CULTURE
REORIENTATION OF CORTICAL MICROTUBULES IN THE SUB-APICAL REGION DURING TUBERIZATION IN SINGLE-NODE STEM SEGMENTS OF POTATO IN CULTURE
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DOI：
10.1093/oxfordjournals.pcp.a078835
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发表时间：
1995-07-01
影响因子：
4.9
通讯作者：
KIKUTA, Y
中科院分区：
文献类型：
--
作者：
FUJINO, K;KODA, Y;KIKUTA, Y
以马铃薯单节茎段为材料，研究了块茎形成过程中的起始事件。在培养基中添加8%的蔗糖引起侧枝伸长的停止和每个芽的亚顶端区域的肿胀。肿胀首先由侧向细胞扩张引起，然后由平周细胞分裂引起。分裂的细胞然后横向扩展。生长方向的改变伴随着皮质微管（MT）阵列的重新定向，这是通过免疫荧光显微镜监测。在伸长的芽的亚顶端区域的细胞具有突出的横向排列的MT。膨大细胞中的MT相对于茎轴呈纵向排列。终于，MT的阵型彻底乱了。与此相反，在GA（3）处理的片段中，侧芽的伸长继续进行，并且在培养过程中，即使在高浓度（8%）的蔗糖存在下，这种芽的近顶端区域的细胞也保留了明显的MT横向阵列。
The initial events in tuberization were examined in single-node stem segments of potato, in which the tuberization was easily regulated in culture. The addition of 8% sucrose to the culture medium caused the cessation of elongation of lateral shoots and the swelling of the sub-apical region of each shoot. Swelling was first induced by lateral cell expansion, which was followed by periclinal cell division. The divided cells then expanded laterally. The alteration in the direction of growth was accompanied by the reorientation of arrays of cortical microtubules (MTs), which was monitored by immunofluorescence microscopy. Cells in the sub-apical region of elongating shoots had prominent transverse arrays of MTs. The MTs in swelling cells were oriented longitudinally with respect to the axis of the shoot. Finally, the arrays of MTs became completely disorganized. By contrast, the elongation of lateral shoots continued in GA(3)-treated segments and the cells in the sub-apical region of such shoots retained conspicuous transverse arrays of MTs during culture, even in the presence of a high concentration (8%) of sucrose.