MATERNAL HORMONAL MANIPULATIONS IN RATS CAUSE OBESITY AND INCREASE MEDIAL HYPOTHALAMIC NOREPINEPHRINE RELEASE IN MALE
MATERNAL HORMONAL MANIPULATIONS IN RATS CAUSE OBESITY AND INCREASE MEDIAL HYPOTHALAMIC NOREPINEPHRINE RELEASE IN MALE
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DOI：
10.1016/0165-3806(95)00078-r
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发表时间：
1995-09-29
期刊：
影响因子：
--
通讯作者：
HOEBEL, BG
中科院分区：
文献类型：
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作者：
JONES, AP;POTHOS, EN;HOEBEL, BG
在先前的工作中，已经表明，在妊娠第15-20天注射鱼精蛋白锌胰岛素或在妊娠前2周营养不良的大鼠的成年雄性而非雌性后代在约50天龄开始出现明显的肥胖。本实验研究的问题是，患有这两种形式肥胖的大鼠是否在已知影响食物摄入和体重的大脑区域显示神经化学异常。使用标准立体定位程序手术植入21号不锈钢导轴。一周后，将26 ga微透析探针放入内侧下丘脑。在两种实验条件下，从雄性后代收集的透析液中含有显著高于对照组的去甲肾上腺素（NE）水平。似乎除了具有相似的发病时程和肥胖的性别依赖性表达外，这两种模型的特征还在于内侧下丘脑NE升高。由于这种肥胖只出现在雄性中，并且在雄性中睾酮水平迅速上升的时候，并且由于睾酮已被证明可以提高大鼠的食物摄入量和体重，因此我们还研究了性腺重量或循环睾酮水平是否通过我们的操作而差异性地升高。
In previous work it has been shown that adult male, but not female, offspring of rats that have either been injected with Protamine Zinc Insulin on days 15-20 of gestation, or undernourished during the first 2 weeks of gestation, develop significant obesity commencing at about 50 days of age. The present experiment examines the question of whether rats with these two forms of obesity display neurochemical abnormalities in areas of the brain known to influence food intake and body weight. Twenty-one gauge stainless steel guide shafts were surgically implanted using standard stereotaxic procedures. One week later 26 ga microdialysis probes were lowered into the medial hypothalamus. Dialysates collected from male offspring in the two experimental conditions contain significantly higher norepinephrine (NE) levels than did controls. It would appear that in addition to sharing a similar time course of onset and a sex dependent expression of obesity, both of these models are also characterized by elevated medial hypothalamic NE. Since this obesity appears only in males, and at a time when testosterone levels are rapidly rising in males, and since testosterone has been shown to elevate food intake and body weight in rats, we also investigated whether gonadal weights or circulating testosterone levels were differentially elevated by our manipulations.