Membrane compartmentation is required for efficient T cell activation.
Membrane compartmentation is required for efficient T cell activation.
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DOI：
10.1016/s1074-7613(00)80577-4
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发表时间：
1998-06
期刊：
影响因子：
32.4
通讯作者：
R. Xavier;Todd Brennan;Qingqin Li;Christine McCormack;B. Seed
中科院分区：
文献类型：
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作者：
R. Xavier;Todd Brennan;Qingqin Li;Christine McCormack;B. Seed
哺乳动物细胞的质膜含有富含鞘糖脂和胆固醇的耐洗涤剂膜筏。虽然已经发现了几个重要的信号分子在这样的筏，证据证明其本地化的功能作用一直很少。使用分馏方案，保留酪氨酸磷酸化，我们表明，T细胞活化导致一个显着的区室化的筏活化的T细胞受体和相关的信号转导分子。通过分散其内容物或通过迫使其内化可逆地破坏筏结构的条件可逆地破坏T细胞活化的最早步骤。因此，筏完整性是有效的T细胞受体信号转导的先决条件。
The plasma membrane of mammalian cells contains detergent-resistant membrane rafts enriched in glycosphingolipids and cholesterol. Although several important signaling molecules have been found in such rafts, evidence documenting a functional role for their localization has been scarce. Using a fractionation scheme that preserves tyrosine phosphorylation, we show that T cell activation leads to a striking compartmentation in the rafts of activated T cell receptor and associated signal-transducing molecules. Conditions that reversibly disrupt raft structure either by dispersing their contents or by forcing their internalization reversibly disrupt the earliest steps of T cell activation. Thus, raft integrity is a prerequisite for efficient T cell receptor signal transduction.