Paradoxical cytoskeleton and microparticle formation changes in monocytes and polymorphonuclear leukocytes in severe systemic inflammatory response syndrome patients
Paradoxical cytoskeleton and microparticle formation changes in monocytes and polymorphonuclear leukocytes in severe systemic inflammatory response syndrome patients
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DOI：
10.1097/01.ta.0000096663.21402.5c
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发表时间：
2003-12-01
影响因子：
--
通讯作者：
Sugimoto, H
中科院分区：
文献类型：
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作者：
Itakura, Y;Ogura, H;Sugimoto, H
背景资料。循环单核细胞和中性粒细胞被认为是控制严重创伤后全身炎症反应的中枢调节细胞。最近有报道称，激活的单核细胞和中性粒细胞在体外可以产生微粒(MPS)。本研究的目的是评价重症全身炎症反应综合征(SIRS)患者单核细胞MPS的产生和细胞骨架的变化，并与PMNL进行比较。纳入20例重度SIRS患者(SIRS标准和血清C反应蛋白10 mg/dL)和15例健康志愿者。用流式细胞仪检测单核细胞和中性粒细胞在有或无脂多糖或甲硫氨酰-亮氨酰-苯丙氨酸(FMLP)作用下的MP形成和F-肌动蛋白含量。流式细胞术检测单核细胞膜人类白细胞抗原DR和CD64的表达及中性粒细胞中O-2(-)的产生。与正常对照组比较，重度SIRS患者单核细胞经内毒素刺激后单核细胞MP生成明显减少(P<0.05)，而PMNLS经FMLP刺激后单核细胞MP生成明显增加(P<0.05)。患者单核细胞经FMLP治疗前后F-肌动蛋白含量也显著降低(p<；0.05)，而PMNL中F-肌动蛋白含量较正常对照组升高(p<；0.05)。患者单核细胞表面人类白细胞抗原-DR的表达显著降低(p<；0.05)，提示单核细胞的调节作用。PMNL中O-2(-)产生增加(p<；0.05)，表现为PMNL活化。重症SIRS患者外周血单核细胞和中性粒细胞中MP的形成和细胞骨架的变化是矛盾的。
Background. Circulating monocytes and polymorphonuclear leukocytes (PMNLs) are considered as central regulators controlling systemic inflammatory response after severe insults. Recently, activated monocytes and PMNLs have been reported to produce microparticles (MPs) in vitro. The objective of this study was to evaluate production of MPs and changes of cytoskeleton in monocytes from severe systemic inflammatory response syndrome (SIRS) patients, and to compare them with those in PMNLs.Methods. Twenty severe SIRS patients (SIRS criteria and serum C-reactive protein > 10 mg/dL) and 15 healthy volunteers were included. MP formation and F-actin content in monocytes and PMNLs were measured by flow cytometry in the presence or absence of lipopolysaccharide or formylmethionyl-leucyl-phenylalanine (FMLP). The membrane expression of human leukocyte antigen-DR and CD64 in monocytes and O-2(-) production in PMNLs were also measured by flow cytometry.Results. In severe SIRS patients, MP formation with and without lipopolysaccharide in monocytes significantly decreased in comparison with those in normal controls (p < 0.05), whereas those with and without FMLP in PMNLs increased (p < 0.05). F-actin content with and without FMLP in monocytes also significantly decreased in patients (p < 0.05), whereas those in PMNLs increased as compared with normal controls (p < 0.05). The expression of human leukocyte antigen-DR in monocytes significantly decreased in patients (p < 0.05), which indicated monocyte modulation. The O-2(-) production in PMNLs increased in patients (p < 0.05), which showed PMNL activation.Conclusion. The changes of MP formation and cytoskeleton in circulating monocytes and PMNLs were paradoxically different in severe SIRS patients.