Regulation of nerve growth factor biosynthesis by beta-adrenergic receptor activation in astrocytoma cells: a potential role of c-Fos protein.
Regulation of nerve growth factor biosynthesis by beta-adrenergic receptor activation in astrocytoma cells: a potential role of c-Fos protein.
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星形细胞瘤细胞中β-肾上腺素能受体激活调节神经生长因子生物合成：c-Fos 蛋白的潜在作用。
DOI：
10.1073/pnas.86.10.3891
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发表时间：
1989
影响因子：
11.1
通讯作者：
Costa,E
中科院分区：
文献类型：
--
作者：
Mocchetti,I;DeBernardi,MA;Szekely,AM;Alho,H;Brooker,G;Costa,E
在C6-2B大鼠星形细胞瘤细胞系中，研究了刺激β-肾上腺素能受体(BAR)后导致神经生长因子(NGF)产生增加的一连串事件。将这些细胞暴露于BAR激动剂异丙肾上腺素后，可引起下列一系列事件：(1)cAMP含量增加；(2)c-Fos mRNA含量增加；(3)c-Fos蛋白免疫反应在细胞核内积聚；(4)NGF mRNA含量增加。C-Fos信使核糖核酸及其翻译产物的增加是早期事件(分别为15分钟和40分钟)，并先于NGF信使核糖核酸的积累，在3小时达到高峰。由于放线菌酮抑制了异丙肾上腺素诱导的c-Fos蛋白和NGF mRNA的积聚，这两个mRNAs的增加似乎是相互关联的。此外，c-Fos mRNA诱导的阻断剂2-氨基嘌呤可减少刺激bar引起的核c-Fos蛋白和NGF mRNA的积聚。这些结果表明，在C6-2B星形细胞瘤细胞中，c-Fos蛋白的核积聚是BAR刺激诱导NGF mRNA表达所必需的。
The chain of events that results in increased production of nerve growth factor (NGF) following beta-adrenergic receptor (BAR) stimulation has been investigated in the C6-2B rat astrocytoma cell line. Exposure of these cells to the BAR agonist isoproterenol elicits the following cascade of events: (i) increase of cAMP content; (ii) increase of c-Fos mRNA content; (iii) accumulation of c-Fos protein immunoreactivity in the nucleus; (iv) increase of NGF mRNA content. The increase in c-Fos mRNA and its translation product are early events (15 and 40 min, respectively) and precede the accumulation of NGF mRNA, which peaks at 3 hr. The increase in the two mRNAs appears interrelated because cycloheximide inhibits the accumulation of c-Fos protein and NGF mRNA elicited by isoproterenol. Moreover, the accumulation of nuclear c-Fos protein and NGF mRNA induced by BAR stimulation is reduced by 2-aminopurine, an inhibitor of c-Fos mRNA induction. These data suggest that, in C6-2B astrocytoma cells, the nuclear accumulation of c-Fos protein is required for the induction of NGF mRNA expression by BAR stimulation.