Level of maternal respiratory syncytial virus (RSV) F antibodies in hospitalized children and correlates of protection.
Level of maternal respiratory syncytial virus (RSV) F antibodies in hospitalized children and correlates of protection.
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住院儿童的母体呼吸道合胞病毒 (RSV) F 抗体水平与保护的相关性。
DOI：
10.1016/j.ijid.2021.06.015
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Yassine,HadiM
中科院分区：
文献类型：
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作者：
Taleb,SaraA;Al-Ansari,Khalid;Nasrallah,GheyathK;Elrayess,MohamedA;Al-Thani,AsmaaA;Derrien-Colemyn,Alexandrine;Ruckwardt,TracyJ;Graham,BarneyS;Yassine,HadiM
研究背景呼吸道合胞病毒（Respiratory syncytial virus，RSV）是引起儿童下呼吸道感染的主要病原，目前尚无有效的疫苗。融合（F）蛋白的稳定形式-前F-是针对不同人群（包括孕妇）的主要候选疫苗。本研究的目的是确定的大小和性质的RSV-directed母源抗体（matAbs）在住院儿童RSV infection. MethodsSixty 5配对的血液样本收集RSV感染的儿童年龄<6个月，他们相应的母亲。使用ELISA筛选所有配对的前F和后F抗体水平。中和抗体（NAbs）在这两个群体中measureuredin vitroagainst mKate RSV-A2 using H28 cells.ResultsIt被发现，14%的matAbs（log212.8）是目前在婴儿住院治疗，平均log 2 EP滴度为10.2针对两个F-蛋白构象。此外，61.4%的母体NAb（log 2 EC 50 = 9.4）在婴儿（log 2 EC 50 = 8.7）中检测到，其中大多数是前F排除（81%）。儿童中的Pre-F抗体与matAbs滴度呈正相关，与年龄和细支气管炎评分呈负相关。结论相对于母体滴度，婴儿中和活性的维持大于基于ELISA的抗体结合的维持，这表明更高效价的抗体可能比弱中和抗体具有更长的半衰期。
BackgroundRespiratory syncytial virus (RSV) is a major cause of lower respiratory infection among children and no vaccine is available. The stabilized form of the fusion (F) protein – pre-F – is a leading vaccine candidate to target different populations, including pregnant women. This study aimed to determine the magnitude and nature of RSV-directed maternal antibodies (matAbs) in hospitalized children with RSV infection.MethodsSixty-five paired blood samples were collected from RSV-infected children aged <6 months and their corresponding mothers. All pairs were screened for levels of pre-F and post-F antibodies using ELISA. The neutralizing antibodies (NAbs) in both groups were measuredin vitroagainst mKate RSV-A2 using H28 cells.ResultsIt was found that 14% of matAbs (log212.8) were present in infants at hospitalization, with an average log2EP titer of 10.2 directed to both F-protein conformations. Additionally, 61.4% of maternal NAbs (log2EC50= 9.4) were detected in infants (log2EC50= 8.7), which were mostly pre-F exclusive (81%). Pre-F antibodies in children showed a positive correlation with matAbs titers and negative correlations with age and bronchiolitis score.ConclusionsThe maintenance of neutralizing activity in infants relative to maternal titers was greater than the maintenance of antibody binding based on ELISA, suggesting that higher-potency antibodies may have a longer half-life than weakly neutralizing antibodies.