TIMP1 mRNA in tumor-educated platelets is diagnostic biomarker for colorectal cancer
TIMP1 mRNA in tumor-educated platelets is diagnostic biomarker for colorectal cancer
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肿瘤培养血小板中的 TIMP1 mRNA 是结直肠癌的诊断生物标志物
DOI：
10.18632/aging.102366
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发表时间：
2019-10-31
期刊：
影响因子：
5.2
通讯作者：
Zhong, Jian
中科院分区：
文献类型：
--
作者：
Yang, Liu;Jiang, Qian;Zhong, Jian
血小板已被证明可以促进肿瘤的生长，包括结肠直肠癌。肿瘤诱导的血小板RNA谱具有癌症诊断的可能性。我们使用RNA测序来鉴定结直肠癌患者和健康志愿者血小板中的基因表达特征。然后，我们使用定量逆转录聚合酶链反应在两步病例对照研究中验证了从RNA测序中选择的生物标志物。我们发现结直肠癌患者的血小板中TIMP 1 mRNA水平高于健康志愿者和炎症性肠病患者的血小板。此外，来自结直肠癌患者的血小板中表达的TIMP 1 mRNA可以被携带到结直肠癌细胞中，在那里它促进体内和体外肿瘤生长。这些发现表明血小板中的TIMP 1 mRNA是结直肠癌的潜在独立诊断生物标志物，并且血小板可以携带RNA进入结直肠癌细胞以促进结直肠癌的发展。
Platelets have been shown to promote the growth of tumors, including colorectal cancer. The RNA profile of tumor-educated platelets has the possibility for cancer diagnosis. We used RNA sequencing to identified the gene expression signature in platelets from colorectal cancer patients and healthy volunteers. We then verified the selected biomarkers from the RNA sequencing in a two-step case-control study using quantitative reverse-transcription polymerase chain reaction. We found that TIMP1 mRNA levels are higher in platelets from colorectal cancer patients than in platelets from healthy volunteers and patients with inflammatory bowel diseases. Additionally, TIMP1 mRNA expressed in platelets from colorectal cancer patients can be carried into colorectal cancer cells, where it promotes tumor growth in vivo and in vitro. These findings show that the TIMP1 mRNA in platelets is a potential independent diagnostic biomarker for colorectal cancer, and that platelets can carry RNAs into colorectal cancer cells to promote colorectal cancer development.