CALCIUM GRADIENTS UNDERLYING POLARIZATION AND CHEMOTAXIS OF EOSINOPHILS
CALCIUM GRADIENTS UNDERLYING POLARIZATION AND CHEMOTAXIS OF EOSINOPHILS
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DOI：
10.1126/science.1948048
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发表时间：
1991-11-01
期刊：
影响因子：
56.9
通讯作者：
FAY, FS
中科院分区：
文献类型：
--
作者：
BRUNDAGE, RA;FOGARTY, KE;FAY, FS
在趋化过程中，通过用改良的数字成像显微镜监测钙敏感染料Fura-2的荧光来成像极化嗜酸性粒细胞中细胞内游离钙（[Ca 2 +]i）的浓度。趋化刺激引起[Ca 2 +]i以与细胞活性相关的非均匀方式增加。在持续向一个方向运动的细胞中，[Ca 2 +]i在细胞后部最高，在细胞前部最低。在细胞转向前，[Ca ~（2+）]i短暂升高。形成新前缘的细胞区域的[Ca 2 +]i最低。[Ca 2 +]i的这些变化为理解被认为是许多细胞定向迁移的基础的细胞骨架蛋白的组织和局部活性提供了基础。
The concentration of intracellular free calcium ([Ca2+]i) in polarized eosinophils was imaged during chemotaxis by monitoring fluorescence of the calcium-sensitive dye Fura-2 with a modified digital imaging microscope. Chemotactic stimuli caused [Ca2+]i to increase in a nonuniform manner that was related to cell activity. In cells moving persistently in one direction, [Ca2+]i was highest at the rear and lowest at the front of the cell. Before cells turned, [Ca2+]i transiently increased. The region of the cell that became the new leading edge had the lowest [Ca2+]i. These changes in [Ca2+]i provide a basis for understanding the organization and local activity of cytoskeletal proteins thought to underlie the directed migration of many cells.