Distinct serotonergic pathways to the amygdala underlie separate behavioral features of anxiety
Distinct serotonergic pathways to the amygdala underlie separate behavioral features of anxiety
复制标题
通往杏仁核的不同血清素通路是焦虑的不同行为特征的基础
DOI：
10.1038/s41593-022-01200-8
复制
发表时间：
2022-12-01
影响因子：
25
通讯作者：
Li，Xiao-Ming
中科院分区：
文献类型：
--
作者：
Yu，Xiao-Dan;Zhu，Yi;Li，Xiao-Ming
小鼠的类痴呆行为包括社交回避和回避明亮的空间。这些特征是否受到明显的调控尚不清楚。在小鼠中，社交刺激和焦虑刺激分别增加和减少基底杏仁核（BA）中5-HT水平。在中缝背核（DRN），投射到BA小清蛋白的5-HT-β vGluT 3神经元（DRN 5-HT-β vGluT 3-BAPV）和锥体神经元（DRN 5-HT-β vGluT 3-BAPyr）具有不同的内在特性和基因表达，并分别对焦虑刺激和社交刺激产生反应。DRN 5-HT → Glu 3 → BAPV的激活抑制了BA中GABA能末梢上5-HT通过GABA受体的释放，诱导了社交回避和对明亮空间的回避。DRN 5-HT受体VGluT 3 →BA神经元的激活通过5-HT 1A受体（HTR 1A）和5-HT 1B受体（HTR 1B）抑制两个亚型的BAP γ神经元。BA中HTR 1A和HTR 1B的药理学抑制分别诱导对明亮空间的回避和社交回避。这些发现突出了从DRN到BA亚群的异源输入在调节单独的焦虑相关行为中的功能意义。
Anxiety-like behaviors in mice include social avoidance and avoidance of bright spaces. Whether these features are distinctly regulated is unclear. We demonstrate that in mice, social and anxiogenic stimuli, respectively, increase and decrease serotonin (5-HT) levels in basal amygdala (BA). In dorsal raphe nucleus (DRN), 5-HT∩vGluT3 neurons projecting to BA parvalbumin (DRN5-HT∩vGluT3-BAPV) and pyramidal (DRN5-HT∩vGluT3-BAPyr) neurons have distinct intrinsic properties and gene expression and respond to anxiogenic and social stimuli, respectively. Activation of DRN5-HT∩vGluT3→BAPVinhibits 5-HT release via GABABreceptors on serotonergic terminals in BA, inducing social avoidance and avoidance of bright spaces. Activation of DRN5-HT∩vGluT3→BA neurons inhibits two subsets of BAPyrneurons via 5-HT1A receptors (HTR1A) and 5-HT1B receptors (HTR1B). Pharmacological inhibition of HTR1A and HTR1B in BA induces avoidance of bright spaces and social avoidance, respectively. These findings highlight the functional significance of heterogenic inputs from DRN to BA subpopulations in the regulation of separate anxiety-related behaviors.