Monomer zinc phthalocyanine/upconversion nanoparticle coated with hyaluronic acid crosslinked gel as NIR light-activated drug for in vitro photodynamic therapy
Monomer zinc phthalocyanine/upconversion nanoparticle coated with hyaluronic acid crosslinked gel as NIR light-activated drug for in vitro photodynamic therapy
复制标题
单体酞菁锌/透明质酸交联凝胶包覆的上转换纳米粒子作为近红外光激活药物用于体外光动力治疗
DOI：
10.1039/c6dt01929c
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发表时间：
2016
影响因子：
4
通讯作者：
Wei Shaohua
中科院分区：
文献类型：
--
作者：
Zhou Lin;Chen Enyi;Jin Weiwei;Wang Yue;Zhou Jiahong;Wei Shaohua
羧基修饰的酞菁锌（ZnPc）是上转换纳米粒子（UCNPs）介导的光动力学疗法（PDT）中经典且广泛使用的光敏剂（PS）。为了提高ZnPc/UCNPs复合体系的光动力学活性，设计并制备了多种具有高红光发射强度的UCNPs。ZnPc-（COOH）4在水中和生理条件下都有聚集的倾向，这会使其PDT活性急剧下降。因此，选择羧基修饰的酞菁锌作为PS是提高其活性的另一种途径。本文合成了三种含4个（ZnPc-（COOH）4）、8个（ZnPc-（COOH）8）和16个（ZnPc-（COOH）16）COOH基团的酞菁锌配合物。结果的比较表明，ZnPc-（COOH）16在生理条件下以其单体形式存在，因为其大的取代基。此外，当与ZnPc-（COOH）4和ZnPc-（COOH）8相比时，ZnPc-（COOH）16显示出上级的单线态氧（1 O2）产生能力。因此，我们选择ZnPc-（COOH）16作为PS用于吸附在UCNP表面上。然后，它们被交联的甲基丙烯酸化透明质酸（m-HA）包裹，m-HA通过结合癌细胞表面上其过表达的受体来提供主动的肿瘤积聚能力。所得到的纳米颗粒可以被癌细胞有效地吸收，并在体外显示出强的近红外（NIR）光触发的PDT。
Carboxyl group modified zinc phthalocyanines (ZnPc) are classic and widely used photosensitizers (PSs) in upconversion nanoparticles (UCNPs) mediated photodynamic therapy (PDT) for tumor treatment. To improve the PDT activity of the complex system of ZnPc and UCNPs, many UCNPs with high red emission intensity were design and prepared. ZnPc-(COOH)4 tends to aggregate both in water and under physiological conditions, which can sharply decrease its PDT activity. Therefore, choosing monomeric COOH groups modified ZnPc as PSs is an alternative way to improve their activity. In this manuscript, three zinc(II) phthalocyanines, substituted with 4 (ZnPc-(COOH)4), 8 (ZnPc-(COOH)8) and 16 (ZnPc-(COOH)16) COOH groups, were synthesized. A comparison of the results indicated that ZnPc-(COOH)16 existed in its monomeric form under physiological conditions because of its large substituents. Moreover, ZnPc-(COOH)16 showed superior singlet oxygen (1O2) generation ability when compared to ZnPc-(COOH)4 and ZnPc-(COOH)8. Therefore, we chose ZnPc-(COOH)16 as PSs for absorption on the surface of the UCNPs. Then, they were encapsulated by crosslinked methacrylated hyaluronic acid (m-HA), which provides active tumor accumulation ability by binding its overexpressed receptors on the surface of cancer cells. The resulting nanoparticles can be effectively taken up by cancer cells and shows strong near-infrared (NIR) light triggered PDT in vitro.