Individuation of monoclonal anti-HPV16 E7 antibody linear peptide epitope by computational biology
Individuation of monoclonal anti-HPV16 E7 antibody linear peptide epitope by computational biology
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DOI：
10.1016/s0196-9781(01)00539-3
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发表时间：
2001-12-01
期刊：
影响因子：
3
通讯作者：
Mittelman, A
中科院分区：
文献类型：
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作者：
Kanduc, D;Lucchese, A;Mittelman, A
我们应用计算生物学来鉴定针对全长HPV 16 E7癌蛋白的小鼠单克隆抗体所识别的线性氨基酸序列。计算机辅助搜索表位肽使用两个参数：E7肽结合MHC II类分子的能力，以及癌蛋白序列与小鼠蛋白质组的相似性水平。我们报告，抗E7单克隆抗体识别的肽具有高的结合潜力的MHC II分子和低水平的分子模拟小鼠蛋白质组。肽与MHC II分子结合的能力似乎是确定肽免疫显性的必要但不充分的条件，因为需要由与宿主蛋白质组的低程度肽相似性支持。(C)2001 Elsevier Science Inc. All rights reserved.
We applied computational biology to identify the linear amino acid sequence recognized by a mouse monoclonal antibody raised against the full length HPV 16 E7 oncoprotein. Computer-assisted search for the epitopic peptide used two parameters: the capability of E7 peptides to bind to MHC class II molecules, and the similarity level of the oncoprotein sequence to the mouse proteome. We report that anti-E7 mAb recognized the peptide having both high binding potential to MHC II molecules and low level of molecular mimicry to mouse proteome. Peptide ability to bind to MHC II molecules appears a necessary but not sufficient condition to determine peptide immunodominance, by needing to be supported by a low degree of peptide, similarity to the host's proteome. (C) 2001 Elsevier Science Inc. All rights reserved.