A bioelectronic taste sensor based on bioengineered Escherichia coli cells combined with ITO-constructed electrochemical sensors.
A bioelectronic taste sensor based on bioengineered Escherichia coli cells combined with ITO-constructed electrochemical sensors.
复制标题
DOI：
10.1016/j.aca.2019.06.023
复制
发表时间：
2019-11
影响因子：
6.2
通讯作者：
Jian Wang;Shu Kong;Fangming Chen;Wei Chen;L. Du;Wen Cai;Liquan Huang;Chunsheng Wu;De-Wen Zhang
中科院分区：
文献类型：
--
作者：
Jian Wang;Shu Kong;Fangming Chen;Wei Chen;L. Du;Wen Cai;Liquan Huang;Chunsheng Wu;De-Wen Zhang
在这项研究中，我们开发了一种新型生物电子味觉传感器，用于检测特定的苦味物质。人类苦味受体 hT2R4 在大肠杆菌 (E. coli) 中高效表达，并被用作主要识别元件。创新性地采用基于ITO的电解质半导体（ES）结构的简单且低成本的电化学装置作为传感器来实时评估受体激活的细菌代谢后果。观察到细胞外酸化率明显增加，这是由于 hT2R4 受体被其靶配体地那铵触发所致。该传感器对 denatonuim 的反应表现出剂量依赖性，范围为 50nM 至 500nM，而没有 hT2R4 受体的非生物工程细菌对相同刺激的反应可以忽略不计。此外，所提出的味道生物传感器的特异性还使用其他典型的苦味物质（例如奎宁和α-萘基硫脲（ANTU））进行了验证。这项研究为构建生物电子味觉传感器提供了一种简单且廉价的方法。
In this study, we developed a novel bioelectronic taste sensor for the detection of specific bitter substances. A human bitter taste receptor, hT2R4, was efficiently expressed inEscherichia coli(E. coli), which was used as the primary recognition element. A simple and low-cost electrochemical device based on ITO-based electrolyte-semiconductor (ES) structure was innovatively employed as the transducer to assess bacterial metabolic consequences of receptor activation in real time. An apparent increase in extracellular acidification rate was observed, which was resulted from the triggering of hT2R4 receptors by their target ligand of denatonium. The sensor showed dose-dependent responses to denatonuim ranging from 50 nM to 500 nM, while non-bioengineered bacteria without hT2R4 receptors exhibited negligible responses to the same stimulus. In addition, the specificity of the proposed taste biosensor was verified using other typical bitter substances such as quinine and alpha-naphthylthiourea (ANTU). This research provides a simple and inexpensive approach for the construction of bioelectronic taste sensors.