Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
复制标题
DOI：
10.1084/jem.166.5.1229
复制
发表时间：
1987-11-01
期刊：
影响因子：
--
通讯作者：
Mosmann TR
中科院分区：
文献类型：
--
作者：
Cherwinski HM;Schumacher JH;Brown KD;Mosmann TR
用mRNA杂交法检测了Con A诱导的11种不同的mRNAs，评价了一组19个T细胞克隆的淋巴因子合成模式。以前描述的两种类型的CD4+Th细胞克隆通过这种方法被清楚地区分开来，两种类型之间的差异现已扩大到六种诱导产物。除少数例外，只有Th1克隆合成了IL-2、干扰素-γ和淋巴毒素的mRNA，只有Th2克隆合成了IL-4、IL-5和另一个诱导基因P600的mRNA。另有4种诱导产物被一种或另一种克隆优先表达，但不是唯一的：GM-CSF、TNF的mRNAs和另一种诱导分泌产物(TY5)在Th1克隆中大量表达，而前脑啡肽原在Th2克隆中优先表达。两种克隆产生的IL-3量相当。用单抗建立了3种检测IL-2、IL-3和IL-4的单抗，并用一种新型抗干扰素单抗XMG1.2建立了检测干扰素-γ的酶联免疫吸附试验。这四种检测方法表明，所有细胞系的分泌蛋白和信使核糖核酸水平都有很好的相关性。本文还讨论了这些发现对正常T细胞的影响。
Lymphokine synthesis patterns of a panel of 19 T cell clones have been evaluated, using mRNA hybridization methods to examine 11 different mRNAs induced by Con A. The two types of CD4+ Th cell clone described previously were clearly distinguished by this procedure, and the differences between the two types have now been extended to six induced products. With minor exceptions, only Th1 clones synthesized mRNA for IL-2, IFN-gamma, and lymphotoxin, and only Th2 clones synthesized mRNA for IL-4, IL-5, and another induced gene, P600. Four more induced products were expressed preferentially but not uniquely by one or another type of clone: mRNAs for GM-CSF, TNF, and another induced, secreted product (TY5) were produced in larger amounts by Th1 clones, whereas preproenkephalin was preferentially expressed by Th2 clones. IL- 3 was produced in similar amounts by both types of clone. mAbs were used to establish three bioassays that were functionally monospecific for IL-2, IL-3, and IL-4, and a new anti-IFN gamma mAb, XMG1.2, was used to establish an ELISA for IFN-gamma. These four assays were used to show that secreted protein and mRNA levels correlated well for all cell lines. The implications of these findings for normal T cells are discussed.