Ultrasound mediated intravesical transfection enhanced by treatment with lidocaine or heat
Ultrasound mediated intravesical transfection enhanced by treatment with lidocaine or heat
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DOI：
10.1097/01.ju.0000139589.52415.3d
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发表时间：
2004-10-01
影响因子：
6.6
通讯作者：
Kondo, T
中科院分区：
文献类型：
--
作者：
Ogawa, R;Kagiya, G;Kondo, T
目的：我们曾报道用利多卡因或加热修饰细胞膜可以增强超声介导的转染率。(USMT)体外作用于PC-3细胞。在本研究中，我们研究了体外培养的人膀胱癌T24细胞株和体内PC-3细胞是否能观察到这种增强。材料和方法：体外转染人T24细胞，用频率为1 MHz、功率为3.6W/cm(2)的超声波(I-SATA)作用20s。以0.78W/cm(I-SATA)的1 MHz超声经腹超声照射膀胱T24或PC-3细胞60s。结果：超声处理T24细胞时，利多卡因或加热处理显著增强了荧光素酶的表达。结果表明，在不同的细胞系中可以实现增强，尽管程度低于PC-3细胞。此外，超声处理后的细胞膜流动性便利化和细胞存活率也不同，可能受细胞膜的不同结构和/或组成的影响。成功地将PC-3和T24细胞导入膀胱腔。结论：USMT及其与利多卡因或加热的增强作用可用于膀胱的基因治疗。
Purpose: We have previously reported that cell membrane modification by lidocaine or heat can enhance ultrasound mediated transfection. (USMT) on PC-3 cells in vitro. In the current study we investigated whether such enhancement could be observed using the T24 human bladder cancer cell line in vitro along with PC-3 in vivo.Materials and Methods: For in vitro transfection T24 cells were sonicated with 1 MHz ultrasound at 3.6 W/cm(2) (I-SATA) for 20 seconds. For in vivo transfection T24 or PC-3 cells in the bladder were transabdominally sonicated with 1 MHz ultrasound at 0.78 W/cm(2) (I-SATA) for 60 seconds. Transfection efficiency was evaluated by the luciferase assay standardized with protein contents of the samples.Results: Lidocaine or heat treatment of T24 cells during sonication enhanced luciferase expression significantly. Results indicated that enhancements could be achieved in a different cell line, although to lesser degrees than with PC-3 cells. In addition, membrane fluidity facilitation and cell viability after sonication were also different, presumably influenced by the different structures and/or compositions of the cell membranes. PC-3 and T24 cells were successfully transfected in the bladder. In addition, USMT enhancements were also observed in the 2 cell lines when sonicated with lidocaine or heat.Conclusions: These results suggest that USMT and its enhancement with lidocaine or heat can be applied for gene therapy in the bladder.