Development of a quantitative gene expression assay for Clamydia trachomatis identified temporal expression of σ factors
Development of a quantitative gene expression assay for Clamydia trachomatis identified temporal expression of σ factors
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DOI：
10.1016/s0014-5793(99)01182-5
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发表时间：
1999-09-24
期刊：
影响因子：
3.5
通讯作者：
Timms, P
中科院分区：
文献类型：
--
作者：
Mathews, SA;Volp, KM;Timms, P
沙眼衣原体是一种重要的人类病原体，具有独特的双相发育周期。我们使用LightCycler方法对沙眼衣原体L2株的基因转录水平进行了定量测定。通过测量16S rRNA转录水平，我们确定沙眼衣原体L2的生成时间约为3h，包涵体破裂大小为200-300个颗粒。衣原体a因子基因rpoD(sigma(66))、rpsD(sigma(28))和rpoN(sigma(54))表现出不同的时间表达模式。RpoD是衣原体早期发育的中心，而rpsD和rpoN在时间上表达，分别与基本体(EB)向网状体(RB)转化和RB向EB转化相一致。(C)1999年欧洲生化学会联合会。
Chlamydia trachomatis is an important human pathogen which possesses a unique bi-phasic developmental cycle. We used lightcycler methodology to quantitatively measure gene transcript levels in C. trachomatis strain L2. By measuring 16S rRNA transcript levels, we determined C. trachomatis L2 to have a generation time of approximately 3 h and an inclusion burst size of 200-300 particles. The three chlamydial a factor genes rpoD (sigma(66)), rpsD (sigma(28)) and rpoN (sigma(54)) exhibited different patterns of temporal expression. rpoD was central to early chlamydial development, whereas rpsD and rpoN were temporally expressed, coinciding with elementary body (EB) to reticulate body (RB) conversion and RB to EB conversion, respectively. (C) 1999 Federation of European Biochemical Societies.