Structural and functional analysis of a growth-regulated gene, the human calcyclin.
Structural and functional analysis of a growth-regulated gene, the human calcyclin.
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DOI：
10.1016/s0021-9258(18)47567-9
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发表时间：
1987-06
期刊：
影响因子：
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通讯作者：
S. Ferrari;B. Calabretta;J. K. Deriel;R. Battini;F. Ghezzo;E. Lauret;C. Griffin;B. Emanuel;F. Gurrieri;R. Baserga
中科院分区：
文献类型：
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作者：
S. Ferrari;B. Calabretta;J. K. Deriel;R. Battini;F. Ghezzo;E. Lauret;C. Griffin;B. Emanuel;F. Gurrieri;R. Baserga
钙周期蛋白最初被定义为 cDNA 克隆 (2A9)，其同源 RNA 具有生长调节作用，其序列与 S-100 蛋白（一种钙结合蛋白）以及酪氨酸激酶主要细胞底物的亚基的序列具有很强的相似性。使用全长 cDNA，我们现在从人类基因组文库中分离出几个含有钙周期蛋白序列的噬菌体。噬菌体之一，ch。 28-10，包含完整的钙周期蛋白基因，以及广泛的侧翼序列。钙周期蛋白基因是一个独特的拷贝基因，有3个外显子。 5' 侧翼序列已在结构和功能上得到表征。除了 TATA 盒外，它在靠近帽位点的区域还包含 GC 盒和与 SV40 启动子的增强子核心具有强同源性的序列。发现其他类似增强子的元件分散在 5' 和 3' 侧翼区域。在转染实验中，邻近的 5' 侧翼区域在驱动连接报告基因的瞬时表达方面非常活跃。最后，钙周期蛋白基因定位于人类 1 号染色体的长臂，靠近滑雪癌基因。
Calcyclin was originally defined as a cDNA clone (2A9) whose cognate RNA is growth-regulated and whose sequence shows strong similarities to the sequences of the S-100 protein, a calcium-binding protein, as well as to a subunit of the major cellular substrate for tyrosine kinase. Using the full-length cDNA, we have now isolated from a human genomic library several phages containing calcyclin sequences. One of the phages, ch. 28-10, contains the entire calcyclin gene, plus extensive flanking sequences. The calcyclin gene is a unique copy gene and has 3 exons. The 5′ flanking sequence has been characterized, both structurally and functionally. Besides a TATA box, it contains, in the region proximate to the cap site, GC boxes and a sequence with a strong homology to the enhancer core of the SV40 promoter. Other enhancer-like elements are found scattered in both the 5′ and 3′ flanking regions. The proximate 5′ flanking region is very active in driving the transient expression of linked reporters in transfection experiments. Finally, the calcyclin gene has been localized to the long arm of human chromosome 1, near the ski oncogene.